Parvalbumin (PVALB) (C-Term) antibody

Details for Product No. ABIN185412
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Antigen
Synonyms pvalb1, pvalb, zgc:92782, MGC107903, zgc:56217, zgc:77798, D22S749, PALB1, Pva, PV, Parv, ATH, PVALB, thymic
Epitope
C-Term
(7), (2), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(37), (31), (26), (12), (12), (12), (2)
Host
Goat
(31), (7), (6), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(28), (16), (12), (11), (10), (7), (2), (2), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN185412
374.27 $
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Immunogen Peptide with sequence C-GVDEFSTLVAES, from the C Terminus of the protein sequence according to NP_002845.1.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Parvalbumin
Background Synonyms: parvalbumin, HGNC:9704, PVALB, D22S749, parvalbumin alpha, MGC116759
Gene ID 5816
NCBI Accession NP_002845
Research Area Neurology, Serum/Plasma Proteins
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 12 kDa band observed in Human Brain lysates (calculated MW of 12.1 kDa according to NP_002845.1). Recommended concentration: 0.1-0.3 µg/mL. Approx 10-12 kDa band observed in rat skeletal muscle and in rat heart cells transfected with human PVALB, but not in untransfected rat heart cells. Data kindly provided by Dr Wang Wang, University of Michigan
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Parvalbumin (PVALB) (C-Term) antibody ABIN185412 (0.1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-Parvalbumin (PVALB) (C-Term) antibody (2) ABIN185412 (0.15µg/ml) staining of adenovirus-mediated gene transfer in rat heart cells 1) Untransfected, 2) transient transfection with Human PVALB. 3) Untransfected rat skeletal muscle. Primary incubation was 1 hour. Detected by chemiluminescence.
General Hashimoto, Bergen, Nguyen et al.: "Relationship of brain-derived neurotrophic factor and its receptor TrkB to altered inhibitory prefrontal circuitry in schizophrenia." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 2, pp. 372-83, 2005 (PubMed).

Validation Images
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