Vacuolar Protein Sorting 45 Homolog (S. Cerevisiae) (VPS45) (Internal Region) antibody

Antigen: Vacuolar Protein Sorting 45 Homolog (S. Cerevisiae) (VPS45)

Synonyms: CG8228, DmelCG8228, dVps45, MENE (3R)-C, MENE(3R)-C, VPS45, Vps45p, ATVPS45, T14N5.2, T14N5_2, VACUOLAR PROTEIN SORTING 45, Vps45, H1, VSP45, VPS45A, VPS45B, VPS54A, VSP45A, H1VPS45, mVps45, AI462172, AW554165, Vsp45a, MGC93104, MGC139503, si:dkey-200h23.3, vps45, MGC146275

Binding Site: Internal Region

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185431

Additional Information

Alternative name: Vps45

Gene ID: 11311

Immunogen: C-FQKKKPKEQQKLES

Description: All names and symbols: VPS45A, HGNC:14579, H1, H1VPS45, VPS45, VPS45B, VPS54A, VSP45, VSP45A , leucocyte vacuolar protein sorting 45, vacuolar protein sorting 45A, vacuolar protein sorting 45A (yeast homolog), vacuolar protein sorting 45B (yeast). Official Symbol: VPS45. RefSeq number(s): NP_009190.2

Specificity: Human, Mouse, Rat, Dog

Synonyms: CG8228, DmelCG8228, dVps45, MENE (3R)-C, MENE(3R)-C, VPS45, Vps45p, ATVPS45, T14N5.2, T14N5_2, VACUOLAR PROTEIN SORTING 45, Vps45, H1, VSP45, VPS45A, VPS45B, VPS54A, VSP45A, H1VPS45, mVps45, AI462172, AW554165, Vsp45a, MGC93104, MGC139503, si:dkey-200h23.3, vps45, MGC146275

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 55kDa band observed in human placenta lysates (calculated MW of 65.1kDa according to NP_009190.2). Recommended concentration: 0.03-0.1 µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185431 (0.03 ug/ml) staining of human placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Nielsen, Christoforidis, Uttenweiler-Joseph et al.: "Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain." in: The Journal of cell biology, Vol. 151, Issue 3, pp. 601-12, 2000 (PubMed).