Rhophilin, rho GTPase Binding Protein 2 (RHPN2) (Internal Region) antibody

Details for Product No. ABIN185439
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms P76RBE, RHOBP, 1300002E07Rik, AA536890, C85860, D7Ertd784e, rhophilin
Epitope
Internal Region
(11), (7), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(18), (3), (3), (2)
Host
Goat
(10), (6), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(12), (11), (5), (2), (2), (1)
Pubmed 1 reference available
Quantity 100 μg
Options
Shipping to United States (Change)
Availability Available
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN185439
374.27 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide with sequence C-TNKNRQKSAST, from the internal region of the protein sequence according to NP_149094.3.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Rhophilin 2
Background Synonyms: RHPN2, p76RBE, rhophilin, Rho GTPase binding protein 2, rhophilin 2, rhophilin-2, RhoB effector, rhophilin-like protein, P76RBE, RHOBP, 76 kDa RhoB effector protein, GTP-Rho-binding protein 2, rhophilin-like Rho-GTPase binding protein
Gene ID 85415
NCBI Accession NP_149094
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 35 kDa band in Human Pancreas and Prostate lysates after 0.3 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 77 kDa according to NP_149094.2. The 35 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Kidney shows staining of cellular membrane and cytoplasm in convoluted tubular cells. Recommended concentration 5-10 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
General Peck, Oberst, Bouker et al.: "The RhoA-binding protein, rhophilin-2, regulates actin cytoskeleton organization." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 43924-32, 2002 (PubMed).

Validation Images
Did you look for something else?
back to top