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Rhophilin, rho GTPase Binding Protein 2 (RHPN2) (Internal Region) antibody

Details for Product No. ABIN185439, Supplier: Login to see
Antigen
  • P76RBE
  • RHOBP
  • 1300002E07Rik
  • AA536890
  • C85860
  • D7Ertd784e
  • rhophilin
Epitope
Internal Region
30
15
10
2
2
1
1
1
1
1
Reactivity
Human
42
5
5
4
1
1
Host
Goat
19
19
4
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
3
3
3
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
32
22
13
6
2
1
Supplier
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Immunogen Peptide with sequence C-TNKNRQKSAST, from the internal region of the protein sequence according to NP_149094.3.
Blocking Peptide Blocking peptide for this product available: ABIN369976
Cross-Reactivity Mouse (Murine), Rat (Rattus), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Rhophilin 2 (RHPN2 Antibody Abstract)
Background Synonyms: RHPN2, p76RBE, rhophilin, Rho GTPase binding protein 2, rhophilin 2, rhophilin-2, RhoB effector, rhophilin-like protein, P76RBE, RHOBP, 76 kDa RhoB effector protein, GTP-Rho-binding protein 2, rhophilin-like Rho-GTPase binding protein
Gene ID 85415
NCBI Accession NP_149094
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 35 kDa band in Human Pancreas and Prostate lysates after 0.3 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 77 kDa according to NP_149094.2. The 35 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Kidney shows staining of cellular membrane and cytoplasm in convoluted tubular cells. Recommended concentration 5-10 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Peck, Oberst, Bouker et al.: "The RhoA-binding protein, rhophilin-2, regulates actin cytoskeleton organization." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 43924-32, 2002 (PubMed).