Rhophilin, rho GTPase Binding Protein 2 (RHPN2) (Internal Region) antibody

Details for Product No. ABIN185439
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Synonyms C85860, AA536890, D7Ertd784e, 1300002E07Rik, RHOBP, P76RBE, rhophilin
Internal Region
(5), (2), (1), (1), (1), (1), (1)
(13), (4), (4), (2)
(7), (5), (2)
Methode Type Peptide ELISA
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(8), (7), (6), (1)
Pubmed 1 reference available
Catalog no. ABIN185439
Quantity 100 µg
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-TNKNRQKSAST, from the internal region of the protein sequence according to NP_149094.3.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Rhophilin 2
Background Synonyms: RHPN2, p76RBE, rhophilin, Rho GTPase binding protein 2, rhophilin 2, rhophilin-2, RhoB effector, rhophilin-like protein, P76RBE, RHOBP, 76 kDa RhoB effector protein, GTP-Rho-binding protein 2, rhophilin-like Rho-GTPase binding protein
Gene ID 85415
NCBI Accession NP_149094.3
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 35 kDa band in Human Pancreas and Prostate lysates after 0.3 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 77 kDa according to NP_149094.2. The 35 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Kidney shows staining of cellular membrane and cytoplasm in convoluted tubular cells. Recommended concentration 5-10 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
General Peck, Oberst, Bouker et al.: "The RhoA-binding protein, rhophilin-2, regulates actin cytoskeleton organization." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 43924-32, 2002 (PubMed).

Hosts (7), (5), (2)
Reactivities (13), (4), (4), (2)
Applications (8), (7), (6), (1)
Epitopes (5), (2), (1), (1), (1), (1), (1)
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