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Rhophilin, rho GTPase Binding Protein 2 (RHPN2) (Internal Region) antibody

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Synonyms P76RBE, RHOBP, 1300002E07Rik, AA536890, C85860, D7Ertd784e, rhophilin
Internal Region
(30), (15), (10), (2), (2), (1), (1), (1), (1), (1)
(42), (5), (5), (4), (1), (1)
(19), (19), (4)
(3), (3), (3), (3), (3), (3)
Methode Type Peptide ELISA
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(32), (22), (13), (6), (2), (1)
Pubmed 1 reference available
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Quantity 100 μg
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Immunogen Peptide with sequence C-TNKNRQKSAST, from the internal region of the protein sequence according to NP_149094.3.
Blocking Peptide Blocking peptide for this product available: ABIN369976
Cross-Reactivity Mouse (Murine), Rat (Rattus), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Rhophilin 2 (RHPN2 Antibody Abstract)
Background Synonyms: RHPN2, p76RBE, rhophilin, Rho GTPase binding protein 2, rhophilin 2, rhophilin-2, RhoB effector, rhophilin-like protein, P76RBE, RHOBP, 76 kDa RhoB effector protein, GTP-Rho-binding protein 2, rhophilin-like Rho-GTPase binding protein
Gene ID 85415
NCBI Accession NP_149094
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 35 kDa band in Human Pancreas and Prostate lysates after 0.3 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 77 kDa according to NP_149094.2. The 35 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Kidney shows staining of cellular membrane and cytoplasm in convoluted tubular cells. Recommended concentration 5-10 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Peck, Oberst, Bouker et al.: "The RhoA-binding protein, rhophilin-2, regulates actin cytoskeleton organization." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 43924-32, 2002 (PubMed).

Catalog No. ABIN185439
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