You are viewing an incomplete version of our website. Please click to reload the website as full version.

Nanog Homeobox (NANOG) (Internal Region) antibody

Details for Product No. ABIN185444, Supplier: Log in to see
Antigen
  • 2410002E02Rik
  • ENK
  • ecat4
Epitope
Internal Region
25
21
19
17
14
14
13
13
13
12
9
8
8
6
5
5
4
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Pig (Porcine)
280
61
34
5
4
2
1
Host
Goat
208
62
36
5
Clonality
Polyclonal
Conjugate
Un-conjugated
14
14
14
12
12
12
3
2
2
2
2
2
2
2
2
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
225
158
83
65
37
37
30
26
22
14
6
4
3
3
2
2
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Immunogen Peptide with sequence C-QNQRMKSKRWQKNN, from the internal region of the protein sequence according to NP_079141.2.
Blocking Peptide Blocking peptide for this product available: ABIN370327
Cross-Reactivity Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name NANOG (NANOG Antibody Abstract)
Background Synonyms: NANOG, Nanog homeobox, HGNC:20857, homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48
Gene ID 79923, 71950, 414065
NCBI Accession NP_079141
Pathways
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 38 kDa band observed in Human Ovary lysates (calculated MW of 34.6 kDa according to NP_079141.1). Recommended concentration: 0.03-0.1 µg/mL.
IHC: This antibody was successfully used on Human Oocytes in Vassena et al, Hum Mol Genet. 2012 Aug 1,21(15):3366-73. PMID: 22547223. Immunofluorescence: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green). See also publication Raya A. et al., Nature. 2009 Jul 2,460(7251):53-9, PMID: 19483674. This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.
Restrictions For Research Use only
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Product cited in: Tolar, McGrath, Xia et al.: "Patient-specific naturally gene-reverted induced pluripotent stem cells in recessive dystrophic epidermolysis bullosa." in: The Journal of investigative dermatology, Vol. 134, Issue 5, pp. 1246-54, 2014 (PubMed).

Montserrat, Ramírez-Bajo, Xia et al.: "Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, OCT4 and SOX2." in: The Journal of biological chemistry, Vol. 287, Issue 29, pp. 24131-8, 2012 (PubMed).

Vassena, Montserrat, Carrasco Canal et al.: "Accumulation of instability in serial differentiation and reprogramming of parthenogenetic human cells." in: Human molecular genetics, Vol. 21, Issue 15, pp. 3366-73, 2012 (PubMed).

Montserrat, de Oñate, Garreta et al.: "Generation of feeder-free pig induced pluripotent stem cells without Pou5f1." in: Cell transplantation, Vol. 21, Issue 5, pp. 815-25, 2012 (PubMed).

Eguizabal, Montserrat, Vassena et al.: "Complete meiosis from human induced pluripotent stem cells." in: Stem cells (Dayton, Ohio), Vol. 29, Issue 8, pp. 1186-95, 2011 (PubMed).

Montserrat, Garreta, González et al.: "Simple generation of human induced pluripotent stem cells using poly-beta-amino esters as the non-viral gene delivery system." in: The Journal of biological chemistry, Vol. 286, Issue 14, pp. 12417-28, 2011 (PubMed).

Montserrat, Bahima, Batlle et al.: "Generation of pig iPS cells: a model for cell therapy." in: Journal of cardiovascular translational research, Vol. 4, Issue 2, pp. 121-30, 2011 (PubMed).

Rodríguez-Pizà, Richaud-Patin, Vassena et al.: "Reprogramming of human fibroblasts to induced pluripotent stem cells under xeno-free conditions." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 1, pp. 36-44, 2010 (PubMed).

Raya, Rodríguez-Pizà, Guenechea et al.: "Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells." in: Nature, Vol. 460, Issue 7251, pp. 53-9, 2009 (PubMed).

Background publications Aasen, Raya, Barrero et al.: "Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes." in: Nature biotechnology, Vol. 26, Issue 11, pp. 1276-84, 2008 (PubMed).

Ralston, Rossant: "Genetic regulation of stem cell origins in the mouse embryo." in: Clinical genetics, Vol. 68, Issue 2, pp. 106-12, 2005 (PubMed).

Skotheim, Lind, Monni et al.: "Differentiation of human embryonal carcinomas in vitro and in vivo reveals expression profiles relevant to normal development." in: Cancer research, Vol. 65, Issue 13, pp. 5588-98, 2005 (PubMed).

Hyslop, Stojkovic, Armstrong et al.: "Downregulation of NANOG induces differentiation of human embryonic stem cells to extraembryonic lineages." in: Stem cells (Dayton, Ohio), Vol. 23, Issue 8, pp. 1035-43, 2005 (PubMed).

Hoei-Hansen, Almstrup, Nielsen et al.: "Stem cell pluripotency factor NANOG is expressed in human fetal gonocytes, testicular carcinoma in situ and germ cell tumours." in: Histopathology, Vol. 47, Issue 1, pp. 48-56, 2005 (PubMed).