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Details for Product No. ABIN185444

Nanog Homeobox (NANOG) (Internal Region) antibody

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Antigen
Synonyms ENK, ecat4, 2410002E02Rik
Epitope
»Alternatives Internal Region
Reactivity
»Alternatives Human, Pig (Porcine)
Host
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Methode Type Peptide ELISA
Application
»Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 11 references available
Catalog no. ABIN185444
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Availability Ships within 5 Business Days
Immunogen Peptide with sequence C-QNQRMKSKRWQKNN, from the internal region of the protein sequence according to NP_079141.2.
Cross-Reactivity Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name NANOG
Background Synonyms: NANOG, Nanog homeobox, HGNC:20857, homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48
Gene ID 79923, 71950, 414065
NCBI Accession NP_079141.2
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 38 kDa band observed in Human Ovary lysates (calculated MW of 34.6 kDa according to NP_079141.1). Recommended concentration: 0.03-0.1 µg/mL.
IHC: This antibody was successfully used on Human Oocytes in Vassena et al, Hum Mol Genet. 2012 Aug 1,21(15):3366-73. PMID: 22547223. Immunofluorescence: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green). See also publication Raya A. et al., Nature. 2009 Jul 2,460(7251):53-9, PMID: 19483674. This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.
Restrictions For Research Use only
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Product cited in: Aasen, Raya, Barrero et al.: "Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes." in: Nature biotechnology, Vol. 26, Issue 11, pp. 1276-84, 2008 (PubMed).

Raya, Rodríguez-Pizà, Guenechea et al.: "Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells." in: Nature, Vol. 460, Issue 7251, pp. 53-9, 2009 (PubMed).

Rodríguez-Pizà, Richaud-Patin, Vassena et al.: "Reprogramming of human fibroblasts to induced pluripotent stem cells under xeno-free conditions." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 1, pp. 36-44, 2010 (PubMed).

Montserrat, Bahima, Batlle et al.: "Generation of pig iPS cells: a model for cell therapy." in: Journal of cardiovascular translational research, Vol. 4, Issue 2, pp. 121-30, 2011 (PubMed).

Montserrat, Garreta, González et al.: "Simple generation of human induced pluripotent stem cells using poly-beta-amino esters as the non-viral gene delivery system." in: The Journal of biological chemistry, Vol. 286, Issue 14, pp. 12417-28, 2011 (PubMed).

Vassena, Montserrat, Carrasco Canal et al.: "Accumulation of instability in serial differentiation and reprogramming of parthenogenetic human cells." in: Human molecular genetics, Vol. 21, Issue 15, pp. 3366-73, 2012 (PubMed).

Montserrat, Ramírez-Bajo, Xia et al.: "Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, OCT4 and SOX2." in: The Journal of biological chemistry, Vol. 287, Issue 29, pp. 24131-8, 2012 (PubMed).

Hoei-Hansen, Almstrup, Nielsen et al.: "Stem cell pluripotency factor NANOG is expressed in human fetal gonocytes, testicular carcinoma in situ and germ cell tumours." in: Histopathology, Vol. 47, Issue 1, pp. 48-56, 2005 (PubMed).

Hyslop, Stojkovic, Armstrong et al.: "Downregulation of NANOG induces differentiation of human embryonic stem cells to extraembryonic lineages." in: Stem cells (Dayton, Ohio), Vol. 23, Issue 8, pp. 1035-43, 2005 (PubMed).

Skotheim, Lind, Monni et al.: "Differentiation of human embryonal carcinomas in vitro and in vivo reveals expression profiles relevant to normal development." in: Cancer research, Vol. 65, Issue 13, pp. 5588-98, 2005 (PubMed).

Ralston, Rossant: "Genetic regulation of stem cell origins in the mouse embryo." in: Clinical genetics, Vol. 68, Issue 2, pp. 106-12, 2005 (PubMed).

Alternatives for antigen "Nanog Homeobox (NANOG)", type "Antibodies"
Hosts (102), (38), (13), (3)
Reactivities (132), (39), (19), (15), (3), (2), (1), (1)
Applications (131), (83), (38), (32), (21), (14), (13), (10), (6), (6), (1)
Conjugates (7), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (13), (10), (9), (6), (6), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
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