Nanog Homeobox (NANOG) (Internal Region) antibody

Details for Product No. ABIN185444
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Antigen
Synonyms 2410002E02Rik, ENK, ecat4
Epitope
Internal Region
(17), (15), (12), (11), (10), (8), (6), (6), (6), (5), (5), (5), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human, Pig (Porcine)
(166), (42), (20), (14), (3), (1), (1)
Host
Goat
(124), (52), (13), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (6), (6), (5), (5), (5), (2), (2), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(151), (101), (42), (34), (33), (25), (19), (16), (10), (5), (1), (1)
Pubmed 11 references available
Quantity 100 μg
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Catalog No. ABIN185444
374.27 $
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Immunogen Peptide with sequence C-QNQRMKSKRWQKNN, from the internal region of the protein sequence according to NP_079141.2.
Cross-Reactivity Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name NANOG
Background Synonyms: NANOG, Nanog homeobox, HGNC:20857, homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48
Gene ID 79923, 71950, 414065
NCBI Accession NP_079141
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 38 kDa band observed in Human Ovary lysates (calculated MW of 34.6 kDa according to NP_079141.1). Recommended concentration: 0.03-0.1 µg/mL.
IHC: This antibody was successfully used on Human Oocytes in Vassena et al, Hum Mol Genet. 2012 Aug 1,21(15):3366-73. PMID: 22547223. Immunofluorescence: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green). See also publication Raya A. et al., Nature. 2009 Jul 2,460(7251):53-9, PMID: 19483674. This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.
Restrictions For Research Use only
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Product cited in: Aasen, Raya, Barrero et al.: "Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes." in: Nature biotechnology, Vol. 26, Issue 11, pp. 1276-84, 2008 (PubMed).

Raya, Rodríguez-Pizà, Guenechea et al.: "Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells." in: Nature, Vol. 460, Issue 7251, pp. 53-9, 2009 (PubMed).

Rodríguez-Pizà, Richaud-Patin, Vassena et al.: "Reprogramming of human fibroblasts to induced pluripotent stem cells under xeno-free conditions." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 1, pp. 36-44, 2010 (PubMed).

Montserrat, Bahima, Batlle et al.: "Generation of pig iPS cells: a model for cell therapy." in: Journal of cardiovascular translational research, Vol. 4, Issue 2, pp. 121-30, 2011 (PubMed).

Montserrat, Garreta, González et al.: "Simple generation of human induced pluripotent stem cells using poly-beta-amino esters as the non-viral gene delivery system." in: The Journal of biological chemistry, Vol. 286, Issue 14, pp. 12417-28, 2011 (PubMed).

Vassena, Montserrat, Carrasco Canal et al.: "Accumulation of instability in serial differentiation and reprogramming of parthenogenetic human cells." in: Human molecular genetics, Vol. 21, Issue 15, pp. 3366-73, 2012 (PubMed).

Montserrat, Ramírez-Bajo, Xia et al.: "Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, OCT4 and SOX2." in: The Journal of biological chemistry, Vol. 287, Issue 29, pp. 24131-8, 2012 (PubMed).

General Hoei-Hansen, Almstrup, Nielsen et al.: "Stem cell pluripotency factor NANOG is expressed in human fetal gonocytes, testicular carcinoma in situ and germ cell tumours." in: Histopathology, Vol. 47, Issue 1, pp. 48-56, 2005 (PubMed).

Hyslop, Stojkovic, Armstrong et al.: "Downregulation of NANOG induces differentiation of human embryonic stem cells to extraembryonic lineages." in: Stem cells (Dayton, Ohio), Vol. 23, Issue 8, pp. 1035-43, 2005 (PubMed).

Skotheim, Lind, Monni et al.: "Differentiation of human embryonal carcinomas in vitro and in vivo reveals expression profiles relevant to normal development." in: Cancer research, Vol. 65, Issue 13, pp. 5588-98, 2005 (PubMed).

Ralston, Rossant: "Genetic regulation of stem cell origins in the mouse embryo." in: Clinical genetics, Vol. 68, Issue 2, pp. 106-12, 2005 (PubMed).

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