Teratocarcinoma-Derived Growth Factor 1, Member 3 (TDGF1.3) (Internal Region) antibody

Details for Product No. ABIN185456
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Antigen
Epitope
Internal Region
(2)
Reactivity
Mouse (Murine)
(7)
Host
Goat
(6), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(5), (3), (3)
Pubmed 1 reference available
Catalog no. ABIN185456
Quantity 100 µg
Price
261.61 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence HFTGRYCEHDQRRSEC, from the internal region of the protein sequence according to NP_115934.1.
Cross-Reactivity Human, Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CFC1 / Cripto-1
Background Synonyms: CFC1, HTX2, CRYPTIC, cripto, FRL-1, cryptic family 1, cripto-1, cryptic, cryptic gene, MGC133213, CR-1, FLJ77897
Gene ID 55997
NCBI Accession NP_115934.1
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 55 kDa band in Human Breast, Placenta and Testis lysates after 0.1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 24.6 kDa according to NP_115934.1. The 55 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Mouse Embryonic Liver shows staining of the hepatocyte cell membranes. Recommended concentration: 1-2 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Teratocarcinoma-Derived Growth Factor 1, Member 3 (TDGF1.3) (Internal Region) antibody ABIN185456 (2 µg/mL) staining of paraffin embedded Mouse Embryo Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
General Bianco, Strizzi, Ebert et al.: "Role of human cripto-1 in tumor angiogenesis." in: Journal of the National Cancer Institute, Vol. 97, Issue 2, pp. 132-41, 2005 (PubMed).

Hosts (6), (1), (1)
Reactivities (7)
Applications (5), (3), (3)
Conjugates (1)
Epitopes (2)
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