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Teratocarcinoma-Derived Growth Factor 1, Member 3 (TDGF1.3) (Internal Region) antibody

Details for Product No. ABIN185456, Supplier: Log in to see
Antigen
Epitope
Internal Region
4
2
1
Reactivity
Mouse (Murine)
11
3
1
Host
Goat
8
3
3
Clonality
Polyclonal
Conjugate
Un-conjugated
2
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
10
6
2
2
1
1
1
Supplier
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Immunogen Peptide with sequence HFTGRYCEHDQRRSEC, from the internal region of the protein sequence according to NP_115934.1.
Blocking Peptide Blocking peptide for this product available: ABIN370181
Cross-Reactivity Human, Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CFC1 / Cripto-1
Background Synonyms: CFC1, HTX2, CRYPTIC, cripto, FRL-1, cryptic family 1, cripto-1, cryptic, cryptic gene, MGC133213, CR-1, FLJ77897
Gene ID 55997
NCBI Accession NP_115934
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 55 kDa band in Human Breast, Placenta and Testis lysates after 0.1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 24.6 kDa according to NP_115934.1. The 55 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Mouse Embryonic Liver shows staining of the hepatocyte cell membranes. Recommended concentration: 1-2 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Immunohistochemistry (IHC) image for anti-Teratocarcinoma-Derived Growth Factor 1, Member 3 (TDGF1.3) (Internal Region) antibody (ABIN185456) ABIN185456 (2 µg/mL) staining of paraffin embedded Mouse Embryo Liver. Steamed antige...
Background publications Bianco, Strizzi, Ebert et al.: "Role of human cripto-1 in tumor angiogenesis." in: Journal of the National Cancer Institute, Vol. 97, Issue 2, pp. 132-41, 2005 (PubMed).