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Teratocarcinoma-Derived Growth Factor 1, Member 3 (TDGF1.3) (Internal Region) antibody

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Internal Region
(5), (2), (1)
Mouse (Murine)
(13), (3), (1)
(9), (4), (3)
Methode Type Peptide ELISA
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
(12), (7), (3), (3), (1), (1), (1)
Pubmed 1 reference available
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Quantity 100 μg
Shipping to United States ( )
Availability Available
Immunogen Peptide with sequence HFTGRYCEHDQRRSEC, from the internal region of the protein sequence according to NP_115934.1.
Blocking Peptide Blocking peptide for this product available: ABIN370181
Cross-Reactivity Human, Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CFC1 / Cripto-1
Background Synonyms: CFC1, HTX2, CRYPTIC, cripto, FRL-1, cryptic family 1, cripto-1, cryptic, cryptic gene, MGC133213, CR-1, FLJ77897
Gene ID 55997
NCBI Accession NP_115934
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 55 kDa band in Human Breast, Placenta and Testis lysates after 0.1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 24.6 kDa according to NP_115934.1. The 55 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Mouse Embryonic Liver shows staining of the hepatocyte cell membranes. Recommended concentration: 1-2 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Immunohistochemistry (IHC) image for anti-Teratocarcinoma-Derived Growth Factor 1, Member 3 (TDGF1.3) (Internal Region) antibody (ABIN185456) ABIN185456 (2 µg/mL) staining of paraffin embedded Mouse Embryo Liver. Steamed antige...
Background publications Bianco, Strizzi, Ebert et al.: "Role of human cripto-1 in tumor angiogenesis." in: Journal of the National Cancer Institute, Vol. 97, Issue 2, pp. 132-41, 2005 (PubMed).

Catalog No. ABIN185456
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