Ribosomal Protein S19 (RPS19) (Internal Region) antibody

Details for Product No. ABIN185519
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Antigen
Synonyms DBA, DBA1, S19, Dsk3
Epitope
Internal Region
(15), (8), (8), (7), (7), (4), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(53), (13), (13), (2), (1), (1)
Host
Goat
(40), (10), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (3), (3), (3)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(54), (36), (17), (9), (2), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN185519
374.27 $
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Immunogen Peptide with sequence C-KMVEKDQDGGRK, from the internal region of the protein sequence according to NP_001013.1.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name RPS19
Background Synonyms: RPS19, ribosomal protein S19, HGNC:10402, DBA, 40S ribosomal protein S19
Gene ID 6223
NCBI Accession NP_001013
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Application Notes ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 15 kDa band observed in lysates of cell lines Daudi and K562 (calculated MW of 16.1 kDa according to NP_001013.1). Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Ribosomal Protein S19 (RPS19) (Internal Region) antibody ABIN185519 (0.03µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Léger-Silvestre, Caffrey, Dawaliby et al.: "Specific Role for Yeast Homologs of the Diamond Blackfan Anemia-associated Rps19 Protein in Ribosome Synthesis." in: The Journal of biological chemistry, Vol. 280, Issue 46, pp. 38177-85, 2005 (PubMed).

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