Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) (Internal Region) antibody

Details for Product No. ABIN185542
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Antigen
Synonyms Cyp1, PPIA-2, fa93g09, ppia, wu:fa93g09, wu:fb05e11, wu:fb13h02, wu:fb15a05, MGC75715, CypA, PPIA, Cypa, 2700098C05, Cphn, CyP-18, CYPA, CYPH, CYCA, CyP-A, MT-ND1, MTND1, NADH1, ND1, cypA
Epitope
Internal Region
(9), (7), (6), (5), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(76), (44), (39), (17), (16), (16), (4), (3), (1)
Host
Goat
(85), (10), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(11), (6), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(84), (53), (28), (21), (16), (12), (10), (3), (3), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN185542
374.27 $
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Immunogen Peptide with sequence C-ERFGSRNGKTSKK, from the internal region of the protein sequence according to NP_066953.1.
Specificity This product is expected to also recognise PPIAL (NP_001008741.1, GeneID: 388817) which is identical to PPIA (NP_066953.1).
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Cyclophilin A / PPIA
Background Synonyms: PPIA, peptidylprolyl isomerase A (cyclophilin A), HGNC:9253, CYPA, CYPH, MGC12404, T cell cyclophilin, cyclosporin A-binding protein, peptidyl-prolyl cis-trans isomerase A, peptidylprolyl isomerase A, rotamase, MGC117158, MGC23397, PPIase A
Gene ID 5478
NCBI Accession NP_066953
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx.18 kDa band observed in Human Lymph Node lysates (calculated MW 18.0 kDa according to NP_066953.1), Recommended Concetration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) (Internal Region) antibody ABIN185542 (2µg/ml) staining of human lymph node lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Berthoux, Sebastian, Sokolskaja et al.: "Cyclophilin A is required for TRIM5{alpha}-mediated resistance to HIV-1 in Old World monkey cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 41, pp. 14849-53, 2005 (PubMed).

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