Vacuolar Protein Sorting 25 Homolog (S. Cerevisiae) (VPS25) (Internal Region) antibody

Details for Product No. ABIN185544
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms
VPS25, CG14750, Dmel\\CG14750, ESCRT-I, ESCRT-II, dVps25, l(2)44Db, l(2)k08904, vps25, GB16268, derp9, eap20, fap20, DDBDRAFT_0189483, DDBDRAFT_0234026, DDB_0189483, DDB_0234026, 1110020N13Rik, AW1074 ... show more
VPS25, CG14750, Dmel\\CG14750, ESCRT-I, ESCRT-II, dVps25, l(2)44Db, l(2)k08904, vps25, GB16268, derp9, eap20, fap20, DDBDRAFT_0189483, DDBDRAFT_0234026, DDB_0189483, DDB_0234026, 1110020N13Rik, AW107467, D11Wsu68e, im:6898134, zgc:162560, DERP9, EAP20, FAP20 show less
Epitope
Internal Region
(10), (9), (3), (3), (2), (1)
Reactivity
Human
(24), (7), (6)
Host
Goat
(21), (4), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(25), (17), (6), (5), (1)
Pubmed 2 references available
Quantity 100 µg
Options
Shipping to United States (Change)
Availability Available
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN185544
374.27 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide with sequence C-QPNVDTRQKQ, from the internal region of the protein sequence according to NP_115729.1.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name VPS25
Background Synonyms: VPS25, vacuolar protein sorting 25 (yeast), HGNC:28122, DERP9, EAP20, MGC10540, vacuolar protein sorting 25, vacuolar protein sorting 25 homolog (S. cerevisiae), FAP20
Gene ID 84313
NCBI Accession NP_115729
Research Area Signaling
Application Notes ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 20-22 kDa kDa band observed in human duodenum, kidney and liver lysates (calculated MW of 20.7 kDa according to NP_115729.1). Recommended concentration: 0.5-1.5 µg/mL.
IHC: In paraffin embedded human kidney, shows granular cytoplasmic staining of endothelial cells. Recommended concentration: 10 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Vacuolar Protein Sorting 25 Homolog (S. Cerevisiae) (VPS25) (Internal Region) antibody ABIN185544 (0.5 ug/ml) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-Vacuolar Protein Sorting 25 Homolog (S. Cerevisiae) (VPS25) (Internal Region) antibody (2) ABIN185544 (10 ug/ml) staining of paraffin embedded human kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
General Vaccari, Bilder: "The Drosophila tumor suppressor vps25 prevents nonautonomous overproliferation by regulating notch trafficking." in: Developmental cell, Vol. 9, Issue 5, pp. 687-98, 2005 (PubMed).

Thompson, Mathieu, Sung et al.: "Tumor suppressor properties of the ESCRT-II complex component Vps25 in Drosophila." in: Developmental cell, Vol. 9, Issue 5, pp. 711-20, 2005 (PubMed).

Validation Images
Did you look for something else?
back to top