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V-Ets erythroblastosis Virus E26 Oncogene Homolog (Avian) (ERG) (C-Term) antibody

Details for Product No. ABIN185550
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Antigen
Synonyms ERG, p55, erg-3, erg_E, D030036I24Rik, erg, erg_A, zgc:103429
Epitope
C-Term
(8), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(53), (15), (12), (2), (2), (2), (1), (1), (1)
Host
Goat
(28), (22), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(40), (19), (12), (11), (6), (4), (3)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN185550
374.27 $
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Immunogen Peptide with sequence C-PNTRLPTSHMPSH, from the C Terminus of the protein sequence according to NP_891548.1, NP_004440.1, NP_001129626.1, NP_001129627.1, NP_001230358.1|.
Specificity This antibody is expected to recognise all reported isoforms (NP_891548.1, NP_004440.1, NP_001129626.1, NP_001129627.1, NP_001230358.1)
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ERG
Background Synonyms: ERG, v-ets erythroblastosis virus E26 oncogene like (avian), HGNC:3446, erg-3, p55, transcriptional regulator ERG (transforming protein ERG), v-ets avian erythroblastosis virus E26 oncogene related, v-ets erythroblastosis virus E26 oncogene like, v-ets erythroblastosis virus E26 oncogene homolog (avian), OTTHUMP00000115970, OTTHUMP00000208036, TMPRSS2-ERG prostate cancer specific, ets-related
Gene ID 2078, 13876, 170909
NCBI Accession NP_891548.1, NP_004440.1, NP_001129626.1, NP_001129627.1, NP_001230358.1|
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Hematopoietic Progenitors
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments in lysates of cell lines HEK293, A431 and Jurkat gave no specific signal but low background (at antibody concentration up to 1 µg/mL).
IHC: In paraffin embedded Human Prostate shows nuclear staining in secretory cells of the glands. Recommended concentration: 4-8 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-V-Ets erythroblastosis Virus E26 Oncogene Homolog (Avian) (ERG) (C-Term) antibody ABIN185550 (10µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
General Tomlins, Rhodes, Perner et al.: "Recurrent fusion of TMPRSS2 and ETS transcription factor genes in prostate cancer." in: Science (New York, N.Y.), Vol. 310, Issue 5748, pp. 644-8, 2005 (PubMed).

Validation Images
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