Phosphodiesterase 4B (PDE4B) (C-Term) antibody

Details for Product No. ABIN185574
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Antigen
Synonyms pde4b, fj87g04, wu:fj87g04, Dpde4, R74983, dunce, PDE4/IVb, Pde4B1, Pde4B2, Pde4B3, Pde4B4
Epitope
C-Term
(5), (1)
Reactivity
Mouse (Murine)
(6), (5), (4), (4), (4)
Host
Goat
(17), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(5)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN185574
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Immunogen Peptide with sequence C-DIDIATEDKSPVDT, from the C Terminus of the protein sequence according to NP_002591.2, NP_001032418.1, NP_001032416.1, NP_001032417.1.
Specificity This antibody is expected to recognise all reported isoforms (NP_002591.2, NP_001032418.1, NP_001032416.1 and NP_001032417.1).
Cross-Reactivity Human, Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Phosphodiesterase 4B
Background Synonyms: PDE4B, phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila), RP5-876C12.1, DKFZp686F2182, DPDE4, MGC126529, PDEIVB, OTTHUMP00000046896, cAMP-specific 3%[yclic phosphodiesterase 4B, dunce-like phosphodiesterase E4, phosphodiesterase 4B, cAMP-specific, phosphodiesterase 4B, cAMP-specific (dunce (Drosophila)-homolog phosphodiesterase E4)
Gene ID 5142
NCBI Accession NP_001032417, NP_001032416, NP_002591, NP_001032418
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 85 kDa band observed in Mouse Brain lysates (calculated MW of 83.3 kDa according to Human NP_002591.2 and 82.1 kDa according to Mouse NP_062814.2). In transfected HEK293 transiently expressing Human PDE4B a band of approx. 85 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.0 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Phosphodiesterase 4B (PDE4B) (C-Term) antibody ABIN185574 (0.5µg/ml) staining of Mouse Brain Lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Millar, Pickard, Mackie et al.: "DISC1 and PDE4B are interacting genetic factors in schizophrenia that regulate cAMP signaling." in: Science (New York, N.Y.), Vol. 310, Issue 5751, pp. 1187-91, 2005 (PubMed).

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