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EN2 antibody (C-Term)

Verified EN2 Reactivity: Human, Mouse WB, ELISA, IF Host: Goat Polyclonal unconjugated
Catalog No. ABIN185576
  • Target See all EN2 Antibodies
    EN2 (Engrailed Homeobox 2 (EN2))
    Binding Specificity
    • 18
    • 15
    • 12
    • 5
    • 1
    C-Term
    Reactivity
    • 56
    • 33
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 54
    • 2
    Goat
    Clonality
    • 54
    • 2
    Polyclonal
    Conjugate
    • 23
    • 6
    • 5
    • 5
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This EN2 antibody is un-conjugated
    Application
    • 50
    • 30
    • 13
    • 13
    • 9
    • 8
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF)
    Purpose
    Engrailed-2
    Sequence
    NHSTTAKEGK SDSE
    Cross-Reactivity
    Dog, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-NHSTTAKEGKSDSE, from the C Terminus of the protein sequence according to NP_001418.2.
    Isotype
    IgG
    Top Product
    Discover our top product EN2 Primary Antibody
  • Application Notes

    Western Blot: Approx 35 kDa band observed in Mouse Brain lysates (calculated MW of f 33.8 kDa according to Mouse NP_034264.1). Recommended concentration: 0.1-1 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:4000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the nuclei of HEK293 and U2OS cells. Recommended concentration: 10μg/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    EN2 (Engrailed Homeobox 2 (EN2))
    Alternative Name
    EN2 (EN2 Products)
    Synonyms
    EN2 antibody, en antibody, en2 antibody, BB131122 antibody, En-2 antibody, RGD1561842 antibody, Gg-En-2 antibody, engrailed homeobox 2 antibody, engrailed homeobox antibody, engrailed 2 antibody, EN2 antibody, en antibody, En2 antibody
    Background
    Engrailed-2, engrailed homolog 2 , EN2, AUTS1
    Gene ID
    2020
    NCBI Accession
    NP_001418
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