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Engrailed Homeobox 2 (EN2) (C-Term) antibody

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Synonyms EN2, en, en2, BB131122, En-2, RGD1561842, Gg-En-2
(53), (5), (2), (1), (1), (1), (1)
Rat (Rattus)
(68), (27), (25), (2), (1), (1)
(61), (15), (4)
(6), (6), (6), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
ELISA, Western Blotting (WB)
(64), (48), (11), (9), (9), (4), (3), (1), (1), (1)
Pubmed 1 reference available
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Quantity 100 μg
Shipping to United States ( )
Availability Available
Immunogen Peptide with sequence C-NHSTTAKEGKSDSE, from the C Terminus of the protein sequence according to NP_001418.2.
Blocking Peptide Blocking peptide for this product available: ABIN370319
Cross-Reactivity Human, Mouse (Murine), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Engrailed-2 (EN2 Antibody Abstract)
Background Synonyms: Engrailed-2, engrailed homolog 2, EN2, AUTS1
Gene ID 2020
NCBI Accession NP_001418
Application Notes ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx. 30 kDa band observed in Mouse and Rat Brain lysates (calculated MW of 34.2 kDa according to Human NP_001418.2 and of 33.8 kDa according to Mouse NP_034264.1 and of 33.7 kDa according to Rat NP_001102684.1). Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Engrailed Homeobox 2 (EN2) (C-Term) antibody (ABIN185576) EB07120 (0.1µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primar...
Background publications Benayed, Gharani, Rossman et al.: "Support for the homeobox transcription factor gene ENGRAILED 2 as an autism spectrum disorder susceptibility locus." in: American journal of human genetics, Vol. 77, Issue 5, pp. 851-68, 2005 (PubMed).

Catalog No. ABIN185576
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