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DCP1 Decapping Enzyme Homolog A (S. Cerevisiae) (DCP1A) (C-Term) antibody

Details for Product No. ABIN185579, Supplier: Login to see
Antigen
  • DCP1A
  • 1110066A22Rik
  • 4930568L04Rik
  • AU019772
  • D14Ertd817e
  • Mitc1
  • SMIF
  • RGD1561298
  • mitc1
  • HSA275986
  • Nbla00360
  • SMAD4IP1
  • smad4ip1
  • smif
Epitope
C-Term
20
15
14
5
5
4
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
82
23
8
4
4
1
1
1
1
1
Host
Goat
53
15
14
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
3
3
3
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
73
51
9
7
6
2
1
1
1
Supplier
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Immunogen Peptide with sequence C-QVLTKNKDNHN, from the C Terminus of the protein sequence according to NP_060873.3.
Blocking Peptide Blocking peptide for this product available: ABIN369724
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name DCP1A (DCP1A Antibody Abstract)
Background Synonyms: DCP1A, decapping enzyme, HSA275986, Nbla00360, SMAD4IP1, SMIF, Smad4-interacting transcriptional co-activator, decapping enzyme hDcp1a, putative protein product of Nbla00360, transcription factor SMIF
Gene ID 55802, 75901
NCBI Accession NP_060873
Pathways
Application Notes ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 75 kDa band observed in human lysates of A431 and HeLa (calculated MW of 63.3 kDa according to NP_060873.3). Recommended concentration: 1-3 µg/mL. An additional band of 48 kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-DCP1 Decapping Enzyme Homolog A (S. Cerevisiae) (DCP1A) (C-Term) antibody (ABIN185579) ABIN185579 (2µg/ml) staining of A431 cell lysate (35µg protein in RIPA buffer). Prima...
Background publications Cougot, Babajko, Séraphin: "Cytoplasmic foci are sites of mRNA decay in human cells." in: The Journal of cell biology, Vol. 165, Issue 1, pp. 31-40, 2004 (PubMed).