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DCP1 Decapping Enzyme Homolog A (S. Cerevisiae) (DCP1A) (C-Term) antibody

Details for Product No. ABIN185579
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Antigen
Synonyms HSA275986, Nbla00360, SMAD4IP1, SMIF, Mitc1, AU019772, D14Ertd817e, 1110066A22Rik, 4930568L04Rik, RGD1561298, mitc1, smad4ip1, smif, DCP1A
Epitope
C-Term
(10), (7), (5), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(61), (30), (16), (14), (14), (12), (1), (1), (1)
Host
Goat
(47), (11), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(50), (31), (10), (6), (4), (3), (3), (2)
Pubmed 1 reference available
Quantity 100 µg
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Availability Available
Catalog No. ABIN185579
374.27 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide with sequence C-QVLTKNKDNHN, from the C Terminus of the protein sequence according to NP_060873.3.
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name DCP1A
Background Synonyms: DCP1A, decapping enzyme, HSA275986, Nbla00360, SMAD4IP1, SMIF, Smad4-interacting transcriptional co-activator, decapping enzyme hDcp1a, putative protein product of Nbla00360, transcription factor SMIF
Gene ID 55802, 75901
NCBI Accession NP_060873.3
Application Notes ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 75 kDa band observed in human lysates of A431 and HeLa (calculated MW of 63.3 kDa according to NP_060873.3). Recommended concentration: 1-3 µg/mL. An additional band of 48 kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-DCP1 Decapping Enzyme Homolog A (S. Cerevisiae) (DCP1A) (C-Term) antibody ABIN185579 (2µg/ml) staining of A431 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Cougot, Babajko, Séraphin: "Cytoplasmic foci are sites of mRNA decay in human cells." in: The Journal of cell biology, Vol. 165, Issue 1, pp. 31-40, 2004 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN185579
374.27 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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