Transcription Factor-Like 5 (Basic Helix-Loop-Helix) (TCFL5) (Internal Region) antibody

Details for Product No. ABIN185720
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Synonyms CHA, E2BP-1, Figlb, bHLHe82, AV260129, RGD1305899, TCFL5, MGC137754
Internal Region
(4), (3), (2), (2), (1), (1), (1), (1)
(19), (8), (2), (1)
(14), (4), (3)
Methode Type Peptide ELISA
ELISA, Western Blotting (WB)
(20), (12), (3), (1)
Pubmed 1 reference available
Catalog no. ABIN185720
Quantity 100 µg
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-KYIQERHGDSLKKE, from the internal region of the protein sequence according to NP_006593.2.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CHA / TCFL5
Background Synonyms: TCFL5, CHA, transcription factor-like 5 (basic helix-loop-helix), E2BP-1, Figlb, MGC46135, HPV-16 E2 binding protein 1, transcription factor-like 5 protein, Cha transcription factor
Gene ID 10732, 277353
NCBI Accession NP_006593.2
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 55 kDa band observed in Human Testis lysates (calculated MW of 52.7 kDa according to NP_006593.2). Recommended concentration: 0.3-1 µg/mL. An additional band of unknown identity was also consistently observed at 28 kDa. This band was greatly reduced by incubation with the immunising peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Transcription Factor-Like 5 (Basic Helix-Loop-Helix) (TCFL5) (Internal Region) antibody ABIN185720 (0.3µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
General Siep, Sleddens-Linkels, Mulders et al.: "Basic helix-loop-helix transcription factor Tcfl5 interacts with the Calmegin gene promoter in mouse spermatogenesis." in: Nucleic acids research, Vol. 32, Issue 21, pp. 6425-36, 2004 (PubMed).

Hosts (14), (4), (3)
Reactivities (19), (8), (2), (1)
Applications (20), (12), (3), (1)
Epitopes (4), (3), (2), (2), (1), (1), (1), (1)
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