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Transcription Factor-Like 5 (Basic Helix-Loop-Helix) (TCFL5) (Internal Region) antibody

Details for Product No. ABIN185720, Supplier: Login to see New
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Synonyms TCFL5, MGC137754, CHA, E2BP-1, Figlb, bHLHe82, AV260129, RGD1305899
Internal Region
(13), (8), (6), (2), (2), (2), (1), (1), (1), (1), (1), (1)
(37), (12), (2), (2), (2), (1), (1)
(27), (7), (4)
(1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
ELISA, Western Blotting (WB)
(35), (22), (8), (2), (1)
Pubmed 1 reference available
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Quantity 100 μg
Shipping to United States ( )
Availability Available
Immunogen Peptide with sequence C-KYIQERHGDSLKKE, from the internal region of the protein sequence according to NP_006593.2.
Blocking Peptide Blocking peptide for this product available: ABIN369395
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CHA / TCFL5 (TCFL5 Antibody Abstract)
Background Synonyms: TCFL5, CHA, transcription factor-like 5 (basic helix-loop-helix), E2BP-1, Figlb, MGC46135, HPV-16 E2 binding protein 1, transcription factor-like 5 protein, Cha transcription factor
Gene ID 10732, 277353
NCBI Accession NP_006593
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 55 kDa band observed in Human Testis lysates (calculated MW of 52.7 kDa according to NP_006593.2). Recommended concentration: 0.3-1 µg/mL. An additional band of unknown identity was also consistently observed at 28 kDa. This band was greatly reduced by incubation with the immunising peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Transcription Factor-Like 5 (Basic Helix-Loop-Helix) (TCFL5) (Internal Region) antibody (ABIN185720) ABIN185720 (0.3µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer) w...
Background publications Siep, Sleddens-Linkels, Mulders et al.: "Basic helix-loop-helix transcription factor Tcfl5 interacts with the Calmegin gene promoter in mouse spermatogenesis." in: Nucleic acids research, Vol. 32, Issue 21, pp. 6425-36, 2004 (PubMed).

Catalog No. ABIN185720
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