PIGB antibody (N-Term)

Catalog No. ABIN1881653

Product Details anti-PIGB Antibody

Target: PIGB (Phosphatidylinositol Glycan Anchor Biosynthesis, Class B (PIGB))

Binding Specificity: AA 28-56, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIGB antibody is un-conjugated

Application: Western Blotting (WB)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This PIGB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-56 amino acids from the N-terminal region of human PIGB.

Clone: RB32518

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-PIGB antibody (ABIN1881653)

Hwang, Hayashi, Kita, Takahashi, Kuge, Naganuma: "siRNA-mediated inhibition of phosphatidylinositol glycan Class B (PIGB) confers resistance to methylmercury in HEK293 cells." in: The Journal of toxicological sciences, Vol. 32, Issue 5, pp. 581-3, (2008) (PubMed).

Anikster, Huizing, Anderson, Fitzpatrick, Klar, Gross-Kieselstein, Berkun, Shazberg, Gahl, Hurvitz: "Evidence that Griscelli syndrome with neurological involvement is caused by mutations in RAB27A, not MYO5A." in: American journal of human genetics, Vol. 71, Issue 2, pp. 407-14, (2002) (PubMed).

Kinoshita, Inoue: "Dissecting and manipulating the pathway for glycosylphos-phatidylinositol-anchor biosynthesis." in: Current opinion in chemical biology, Vol. 4, Issue 6, pp. 632-8, (2001) (PubMed).

Takahashi, Inoue, Ohishi, Maeda, Nakamura, Endo, Fujita, Takeda, Kinoshita: "PIG-B, a membrane protein of the endoplasmic reticulum with a large lumenal domain, is involved in transferring the third mannose of the GPI anchor." in: The EMBO journal, Vol. 15, Issue 16, pp. 4254-61, (1996) (PubMed).

Target Details for PIGB

Target: PIGB (Phosphatidylinositol Glycan Anchor Biosynthesis, Class B (PIGB))

Alternative Name: PIGB (PIGB Products)

Background: This gene encodes a transmembrane protein that is located in the endoplasmic reticulum and is involved in GPI-anchor biosynthesis. The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This gene is thought to encode a member of a family of dolichol-phosphate-mannose (Dol-P-Man) dependent mannosyltransferases.

Molecular Weight: 65056

NCBI Accession: NP_004846

UniProt: Q92521

Pathways: Inositol Metabolic Process