CD48 Molecule (CD48) antibody (APC)

Antigen: CD48 Molecule (CD48)

Synonyms: BCM1, BLAST, hCD48, mCD48, BLAST1, SLAMF2, MEM-102, Bcm-1, Sgp-60, BLAST-1, AI449234, AW610730

Clonality: Monoclonal (MEM-102)

Host: Mouse

Reactivity: Human, Primate

Conjugate: APC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN192135

Additional Information

Alternative name: CD48

Gene ID: 962

Immunogen: Raji human Burkitt's lymphoma cell line

Isotype: IgG1

Clone: MEM-102

Description: CD48 (Blast-1) belongs to the CD2 subset of the Ig superfamily, which includes CD2,CD2F-10, CD58, CD84, CD150, CD229, CD244 and others. These molecules bind to thesame or another members of their family, thus mediate homotypic or heterotypic adhesion.CD48 is a GPI-anchored protein broadly expressed on hematopoietic cells and serves as ahigh affinity ligand for 2B4 and low affinity ligand for CD2. 2B4-CD48 interaction among NKcells and NK-T cells regulates cell proliferation. Signaling through CD48 results in eosinophilactivation and CD48 expression is increased in several infectious diseases.

Specificity: The antibody MEM-102 reacts with CD48 antigen (Blast-1), a 40-47 kDa GPI-anchoredmembrane protein (immunoglobulin supergene family) widely expressed on hematopoieticcells, it is negative on granulocytes, platelets and erythrocytes.HLDA V, WS Code AS S014

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Surface staining of human peripheral blood cells with anti-CD48 (MEM-102) Alexa Fluor

Publications

Koruinek, Stefanovua, Angelisovua et al.: "The human leucocyte antigen CD48 (MEM-102) is closely related to the activation marker Blast-1." in: Immunogenetics, Vol. 33, Issue 2, pp. 108-12, 1991 (PubMed).

Bazil, Stefanovua, Hilgert et al.: "Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102." in: Folia biologica, Vol. 35, Issue 5, pp. 289-97, 1990 (PubMed).

Angelisová, Drbal, Horejsí et al.: "Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase." in: Blood, Vol. 93, Issue 4, pp. 1437-9, 1999 (PubMed).

Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Carlsten, Baumann, Simonsson et al.: "Reduced DNAM-1 expression on bone marrow NK cells associated with impaired killing of CD34(+) blasts in myelodysplastic syndrome." in: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, 2010 (PubMed).