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CD48 Molecule (CD48) antibody (APC)

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Antigen
Synonyms AI449234, AW610730, BCM1, BLAST, BLAST-1, BLAST1, Bcm-1, MEM-102, SLAMF2, Sgp-60, hCD48, mCD48
Reactivity
Human, Non-Human Primate
(168), (105), (37), (8), (5), (4), (1), (1)
Host
Mouse
(154), (53), (42), (36), (10), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
APC
(47), (46), (18), (15), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS)
(216), (80), (47), (31), (28), (25), (22), (13), (8), (7), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 11 references available
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Quantity 100 tests
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Immunogen Raji human Burkitt's lymphoma cell line
Clone MEM-102
Isotype IgG1
Specificity The antibody MEM-102 reacts with CD48 antigen (Blast-1), a 40-47 kDa GPI-anchored membrane protein (immunoglobulin supergene family) widely expressed on hematopoietic cells, it is negative on granulocytes, platelets and erythrocytes.HLDA V, WS Code AS S014
Characteristics The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Alternative Name CD48 (CD48 Antibody Abstract)
Background CD48 (Blast-1) belongs to the CD2 subset of the Ig superfamily, which includes CD2, CD2F-10, CD58, CD84, CD150, CD229, CD244 and others. These molecules bind to the same or another members of their family, thus mediate homotypic or heterotypic adhesion. CD48 is a GPI-anchored protein broadly expressed on hematopoietic cells and serves as a high affinity ligand for 2B4 and low affinity ligand for CD2. 2B4-CD48 interaction among NK cells and NK-T cells regulates cell proliferation. Signaling through CD48 results in eosinophil activation and CD48 expression is increased in several infectious diseases.
Gene ID 962
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 µL reagent / 100 µL of whole blood or 10^6 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Supplier Images
Flow Cytometry (FACS) image for anti-CD48 Molecule (CD48) antibody (APC) (ABIN192135) Surface staining of human peripheral blood cells with anti-CD48 (MEM-102) Alexa Fluor...
Product cited in: Schatzlmaier, Supper, Göschl et al.: "Rapid multiplex analysis of lipid raft components with single-cell resolution." in: Science signaling, Vol. 8, Issue 395, pp. rs11, 2015 (PubMed).

Carlsten, Baumann, Simonsson et al.: "Reduced DNAM-1 expression on bone marrow NK cells associated with impaired killing of CD34(+) blasts in myelodysplastic syndrome." in: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, 2010 (PubMed).

Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Angelisová, Drbal, Horejsí et al.: "Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase." in: Blood, Vol. 93, Issue 4, pp. 1437-9, 1999 (PubMed).

Stulnig, Berger, Sigmund et al.: "Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol." in: The Journal of biological chemistry, Vol. 272, Issue 31, pp. 19242-7, 1997 (PubMed).

Korínek, Stefanová, Angelisová et al.: "The human leucocyte antigen CD48 (MEM-102) is closely related to the activation marker Blast-1." in: Immunogenetics, Vol. 33, Issue 2, pp. 108-12, 1991 (PubMed).

Background publications Munitz, Bachelet, Eliashar et al.: "CD48 is an allergen and IL-3-induced activation molecule on eosinophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 1, pp. 77-83, 2006 (PubMed).

Assarsson, Kambayashi, Persson et al.: "2B4/CD48-mediated regulation of lymphocyte activation and function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 4, pp. 2045-9, 2005 (PubMed).

Mathew, Kumaresan, Lee et al.: "Mutational analysis of the human 2B4 (CD244)/CD48 interaction: Lys68 and Glu70 in the V domain of 2B4 are critical for CD48 binding and functional activation of NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 2, pp. 1005-13, 2005 (PubMed).

Lee, Forman, McNerney et al.: "Requirement of homotypic NK-cell interactions through 2B4(CD244)/CD48 in the generation of NK effector functions." in: Blood, Vol. 107, Issue 8, pp. 3181-8, 2006 (PubMed).

Bazil, Stefanová, Hilgert et al.: "Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102." in: Folia biologica, Vol. 35, Issue 5, pp. 289-97, 1990 (PubMed).

Catalog No. ABIN192135
$334.95
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