Phosphotyrosine antibody

Details for Product No. ABIN197635
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Antigen
Reactivity
All Species
(166), (38), (24), (20), (16), (9), (4), (1), (1), (1), (1)
Host
Mouse
(177), (48), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(28), (21), (18), (7), (6), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(168), (109), (91), (34), (32), (27), (20), (15), (15), (10), (3), (1), (1), (1)
Pubmed 4 references available
Quantity 0.5 mg
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN197635
308.00 $
Plus shipping costs $45.00

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  • +1 404 474 4654
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Clone PY20
Isotype IgG2b
Purification Affinity Chromatography
Research Area Signaling, Metabolism, Amino Acids, Protein Modifications
Application Notes ELISA (0.1 ug/ml)ImmunofluorescenceWestern blotting (2g/ml)Immmunoprecipitation: use Protein A and Ab at 2 g/mg protein lysate. Immunohistochemistry on paraffin embedded sections: 5-10 ug/ml 30 min RT (Staining offormalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for10-20 min. followed by cooling at RT for 20 min. )Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Borate buffered saline pH 8.0 0.09% Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 4°C for up to one month or aliquot and store at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Phosphotyrosine antibody anti-Phosphotyrosine antibody
Background publications Ruff-Jamison, Campos-González, Glenney: "Heavy and light chain variable region sequences and antibody properties of anti-phosphotyrosine antibodies reveal both common and distinct features." in: The Journal of biological chemistry, Vol. 266, Issue 10, pp. 6607-13, 1991 (PubMed).

Takagi, Daibata, Last et al.: "Intracellular localization of tyrosine kinase substrates beneath crosslinked surface immunoglobulins in B cells." in: The Journal of experimental medicine, Vol. 174, Issue 2, pp. 381-8, 1991 (PubMed).

Glenney, Zokas, Kamps: "Monoclonal antibodies to phosphotyrosine." in: Journal of immunological methods, Vol. 109, Issue 2, pp. 277-85, 1988 (PubMed).

Vendel, Calemine-Fenaux, Izrael-Tomasevic et al.: "B and T lymphocyte attenuator regulates B cell receptor signaling by targeting Syk and BLNK." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 3, pp. 1509-17, 2009 (PubMed).

Validation Images
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