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Details for Product No. ABIN197635

Phosphotyrosine antibody

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Antigen
Reactivity
»Alternatives All Species
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Pubmed 4 references available
Catalog no. ABIN197635
Quantity 0.5 mg
Price
297.00 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 6 to 8 Business Days
Clone PY20
Isotype IgG2b
Purification Affinity Chromatography
Research Area Signaling, Metabolism, Amino Acids, Protein Modifications
Application Notes ELISA (0.1 ug/ml)ImmunofluorescenceWestern blotting (2g/ml)Immmunoprecipitation: use Protein A and Ab at 2 g/mg protein lysate. Immunohistochemistry on paraffin embedded sections: 5-10 ug/ml 30 min RT (Staining offormalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for10-20 min. followed by cooling at RT for 20 min. )Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Borate buffered saline pH 8.0 0.09% Sodium Azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 4°C for up to one month or aliquot and store at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Ruff-Jamison, Campos-González, Glenney: "Heavy and light chain variable region sequences and antibody properties of anti-phosphotyrosine antibodies reveal both common and distinct features." in: The Journal of biological chemistry, Vol. 266, Issue 10, pp. 6607-13, 1991 (PubMed).

Takagi, Daibata, Last et al.: "Intracellular localization of tyrosine kinase substrates beneath crosslinked surface immunoglobulins in B cells." in: The Journal of experimental medicine, Vol. 174, Issue 2, pp. 381-8, 1991 (PubMed).

Glenney, Zokas, Kamps: "Monoclonal antibodies to phosphotyrosine." in: Journal of immunological methods, Vol. 109, Issue 2, pp. 277-85, 1988 (PubMed).

Vendel, Calemine-Fenaux, Izrael-Tomasevic et al.: "B and T lymphocyte attenuator regulates B cell receptor signaling by targeting Syk and BLNK." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 3, pp. 1509-17, 2009 (PubMed).

Alternatives for antigen "Phosphotyrosine", type "Antibodies"
Hosts (183), (48), (2)
Reactivities (167), (39), (25), (21), (16), (11), (5), (3), (2), (1)
Applications (173), (118), (92), (36), (32), (23), (18), (17), (14), (10), (3), (2), (1), (1), (1), (1)
Conjugates (29), (23), (19), (7), (7), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (8), (3), (2), (1), (1), (1), (1), (1)
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