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Phosphotyrosine antibody

Details for Product No. ABIN197635, Supplier: Log in to see
Antigen
Reactivity
All Species
110
52
43
29
29
27
25
9
9
5
1
Host
Mouse
179
63
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
27
24
17
15
7
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
178
110
85
77
72
51
28
27
11
7
7
2
1
1
1
1
1
Supplier
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Clone PY20
Isotype IgG2b
Purification Affinity Chromatography
Background The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the T-cell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyse the transfer of ATP to a tyrosine residue on specific cell protein targets.
Research Area Signaling, Metabolism, Amino Acids, Protein Modifications
Pathways
Application Notes ELISA (0.1 μg/mL)ImmunofluorescenceWestern blotting (2g/mL)Immmunoprecipitation: use Protein A and Ab at 2 g/mg protein lysate. Immunohistochemistry on paraffin embedded sections: 5-10 μg/mL 30 min RT (Staining offormalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for10-20 min. followed by cooling at RT for 20 min. )
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Borate buffered saline pH 8.0, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 4 °C for up to one month or aliquot and store at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Phosphotyrosine antibody (ABIN197635) anti-Phosphotyrosine antibody
Background publications Vendel, Calemine-Fenaux, Izrael-Tomasevic et al.: "B and T lymphocyte attenuator regulates B cell receptor signaling by targeting Syk and BLNK." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 3, pp. 1509-17, 2009 (PubMed).

Glenney, Zokas, Kamps: "Monoclonal antibodies to phosphotyrosine." in: Journal of immunological methods, Vol. 109, Issue 2, pp. 277-85, 1988 (PubMed).

Takagi, Daibata, Last et al.: "Intracellular localization of tyrosine kinase substrates beneath crosslinked surface immunoglobulins in B cells." in: The Journal of experimental medicine, Vol. 174, Issue 2, pp. 381-8, 1991 (PubMed).

Ruff-Jamison, Campos-González, Glenney: "Heavy and light chain variable region sequences and antibody properties of anti-phosphotyrosine antibodies reveal both common and distinct features." in: The Journal of biological chemistry, Vol. 266, Issue 10, pp. 6607-13, 1991 (PubMed).