F4/80 antibody

Catalog No. ABIN204198

Product Details anti-F4/80 Antibody

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This F4/80 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes a 125kD extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. Cross-reacts with human heart macrophages.

Purification: Purified

Immunogen: Mouse F4/80.

Clone: BM8

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:25), IHC, IHC-Fr, IHC-P, IP, WB (1:25)

Usage: Suitable for use in Western Blot, Flow Cytometry Immunoprecipitation and Immunohistochemistry. Immunohistochemistry (frozen and paraffin sections): 1:25, F4/80 is sensitive to 2-mercaptoethanol. The antibody requires proteinase K treatment of paraffin sections. Flow Cytometry: 1:25. Western Blot: 1:25.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.1 % BSA, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for F4/80

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Alternative Name: ADGRE1 / EMR1 (EMR1 Products)

Background: Name/Gene ID: ADGRE1
Subfamily: Orphan-B
Family: GPCR

Synonyms: ADGRE1, EMR1 hormone receptor, F4/80, EGF-like module receptor 1, TM7LN3, EMR1

Gene ID: 2015

UniProt: Q14246