CD8 antibody

Catalog No. ABIN2145444

Product Details anti-CD8 Antibody

Target: CD8

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD8 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Characteristics: The 4A2.43 monoclonal antibody reacts with the mouse CD8 Molecule which expressed on most thymocytes and mature T lymphocytes Ts / c sub-group cells.CD8 is an antigen co-recepter on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells.CD8 promotes T cells activation through its association with the TRC complex and protei tyrosine kinase lck.

Purification: Purified

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Procedure:

• Take 100 μL a certain amount of prepared cells and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add appropriate amount of fluorescent-labeled anti-Rat IgGs and incubate for 20 minutes away from light at room temperature.
• Repeat step 5.
• Add 0.3-0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined in two hours on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CD8

Target: CD8

Alternative Name: CD8 (CD8 Products)