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Arylsulfatase A (ARSA) (Internal Region) antibody

Details for Product No. ABIN238536
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Antigen
Synonyms ARSA, zgc:101575, arsa, AS-A, ASA, AW212749, As-2, As2, TISP73, MLD, mld
Epitope
Internal Region
(10), (6), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine)
(67), (42), (40), (14), (14), (14), (1)
Host
Goat
(52), (13), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(51), (21), (19), (17), (7), (3), (2), (1)
Pubmed 4 references available
Quantity 100 µg
Options
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Catalog No. ABIN238536
374.27 $
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Immunogen Peptide with sequence C-YDLSKDPGENYN, from the internal region of the protein sequence according to NP_000478.2.
Cross-Reactivity Human, Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Arylsulfatase A
Background Synonyms: ARSA, arylsulfatase A, MLD, cerebroside-sulfatase, cerebroside 3-sulfatase
Gene ID 410, 11883, 315222
NCBI Accession NP_000478.2
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 55 kDa band observed in Mouse Testis lysates (calculated MW of 53.6 kDa according to human NP_000478.2 and 53.7 kDa according to Mouse NP_033843.2). Recommended concentration: 0.5-1.5 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Arylsulfatase A (ARSA) (Internal Region) antibody ABIN238536 (0.5µg/ml) staining of Mouse Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product cited in: Zhang, Nakajima, Kamijo et al.: "Acute kidney injury induced by protein-overload nephropathy down-regulates gene expression of hepatic cerebroside sulfotransferase in mice, resulting in reduction of liver and serum sulfatides." in: Biochemical and biophysical research communications, Vol. 390, Issue 4, pp. 1382-8, 2009 (PubMed).

Kiebish, Young, Lehman et al.: "Chronic caloric restriction attenuates a loss of sulfatide content in PGC-1α-/- mouse cortex: a potential lipidomic role of PGC-1α in neurodegeneration." in: Journal of lipid research, Vol. 53, Issue 2, pp. 273-81, 2012 (PubMed).

Kimura, Nakajima, Kamijo et al.: "Hepatic Cerebroside Sulfotransferase Is Induced by PPARα Activation in Mice." in: PPAR research, Vol. 2012, pp. 174932, 2012 (PubMed).

General Sevin, Benraiss, Van Dam et al.: "Intracerebral adeno-associated virus-mediated gene transfer in rapidly progressive forms of metachromatic leukodystrophy." in: Human molecular genetics, Vol. 15, Issue 1, pp. 53-64, 2005 (PubMed).

Validation Images
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