Potassium Inwardly-Rectifying Channel, Subfamily J, Member 11 (KCNJ11) (N-Term), (Internal Region) antibody

Details for Product No. ABIN238541
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Antigen
Synonyms kir6.2, Kir6.2, mBIR, BIR, HHF2, IKATP, KIR6.2, PHHI, TNDM3
Epitope
N-Term, Internal Region
(22), (22), (14), (9), (7), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(71), (55), (32), (28), (24), (12)
Host
Goat
(66), (12), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(61), (41), (20), (18), (17), (11), (10), (5), (3), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN238541
374.27 $
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Immunogen Peptide with sequence C-AEDPAKPRYRARQ, from the internal region (near the N Terminus) of the protein sequence according to NP_000516.3.
Specificity This antibody is expected to recognise isoform 1 (NP_000516.3) only.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name KCNJ11 / KATP
Background Synonyms: KCNJ11, IKATP, potassium inwardly-rectifying channel, subfamily J, member 11, BIR, HHF2, KIR6.2, MGC133230, PHHI, TNDM3, ATP-sensitive inward rectifier potassium channel 11, beta-cell inward rectifier subunit, inwardly rectifying potassium channel KIR6.2, potassium inwardly-rectifying channel J11
Gene ID 3767
NCBI Accession NP_000516
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 45-48 kDa band observed in Human Skeletal Muscle lysates (calculated MW of 43.5 kDa according to NP_000516.3). Recommended concentration: 0.1-0.3 µg/mL.
IHC: In paraffin embedded Human Pancreas shows variable staining across the islet of Langerhans. Recommended concentration: 3-5 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Potassium Inwardly-Rectifying Channel, Subfamily J, Member 11 (KCNJ11) (N-Term), (Internal Region) antibody ABIN238541 (0.1µg/ml) staining of Human Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Filosa, Bonev, Straub et al.: "Local potassium signaling couples neuronal activity to vasodilation in the brain." in: Nature neuroscience, Vol. 9, Issue 11, pp. 1397-1403, 2006 (PubMed).

Validation Images
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