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Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 6 (SLC12A6) (Internal Region) antibody

Details for Product No. ABIN238578
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Antigen
Synonyms Kcc3, ACCPN, KCC3, KCC3A, KCC3B, 9530023I19Rik, gaxp
Epitope
Internal Region
(8), (7), (4), (3), (2), (1), (1), (1), (1)
Reactivity
Human
(38), (20), (19), (12), (12)
Host
Goat
(35), (3), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(29), (19), (15), (10), (5), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN238578
374.27 $
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Immunogen Peptide with sequence SRHIDVCSKTKE, from the internal region of the protein sequence according to NP_001035959.1, NP_001035960.1, NP_001035961.1, NP_001035962.1, NP_005126.1, NP_598408.1.
Specificity This antibody is expected to recognise all 6 reported isofoms (NP_598408.1, NP_005126.1, NP_001035959.1, NP_001035960.1, NP_001035961.1, NP_001035962.1, ),
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name KCC3 / SLC12A6
Background Synonyms: SLC12A6, KCC3, solute carrier family 12 (potassium/chloride transporters), member 6, ACCPN, DKFZP434D2135, KCC3A, KCC3B, agenesis of corpus callosum and peripheral neuropathy (Andermann syndrome), potassium chloride cotransporter 3, potassium chloride c
Gene ID 9990
NCBI Accession NP_001035959.1, NP_001035960.1, NP_001035961.1, NP_001035962.1, NP_005126.1, NP_598408.1
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 120 kDa band observed in Human Heart and Kidney lysates (calculated MW ranging 121-128 kDa according to NP_598408.1, NP_005126.1, NP_001035959.1, NP_001035960.1, NP_001035961.1, NP_001035962.1). Recommended concentration: 0.1-0.3 µg/mL.
IHC: In paraffin embedded Human Heart shows staining of myocardial fibrils in transverse section. Recommended concentration: 2-4 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 6 (SLC12A6) (Internal Region) antibody ABIN238578 (0.1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Simard, Bergeron, Frenette-Cotton et al.: "Homooligomeric and heterooligomeric associations between K+-Cl- cotransporter isoforms and between K+-Cl- and Na+-K+-Cl- cotransporters." in: The Journal of biological chemistry, Vol. 282, Issue 25, pp. 18083-93, 2007 (PubMed).

Validation Images
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