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WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (C-Term) antibody

Details for Product No. ABIN238610
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Antigen
Synonyms WISP1, si:ch211-38f10.2, CCN4, WISP1c, WISP1i, WISP1tc, AW146261, Elm1, WISP-1
Epitope
C-Term
(8), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(41), (22), (17)
Host
Goat
(38), (4), (3), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(32), (24), (14), (10), (9), (5), (3), (2), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN238610
374.27 $
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Immunogen Peptide with sequence C-ESYPDFSEIAN, from the C Terminus of the protein sequence according to NP_003873.1, NP_543028.1.
Specificity This antibody is expected to recognize both reported isoforms (NP_003873.1, NP_543028.1).
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name WISP1
Background Synonyms: WISP1, WNT1 inducible signaling pathway protein 1, CCN4, WISP1c, WISP1i, WISP1tc, WNT1 induced secreted protein 1, Wnt-1 inducible signaling pathway protein 1, wnt-1 signaling pathway protein 1
Gene ID 8840, 22402, 65154
NCBI Accession NP_003873.1, NP_543028.1
Research Area Stem Cells, Wnt, Transcription Factors, DNA/RNA
Application Notes ELISA: antibody detection limit dilution 1:128000.
Western blot: In transfected HEK293 transiently expressing WISP1 a band of approx. 35 kDa is observed. This band is not observed in the non-transfected HEK293. . Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (C-Term) antibody ABIN238610 (0.1 ug/ml) staining of HEK293 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Major, Camp, Berndt et al.: "Wilms tumor suppressor WTX negatively regulates WNT/beta-catenin signaling." in: Science (New York, N.Y.), Vol. 316, Issue 5827, pp. 1043-6, 2007 (PubMed).

Validation Images
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