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WISP1 antibody (C-Term)

Verified WISP1 Reactivity: Human WB, ELISA, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN238610
  • Target See all WISP1 Antibodies
    WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
    Binding Specificity
    • 15
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 40
    • 26
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 55
    • 5
    • 1
    Goat
    Clonality
    • 57
    • 4
    Polyclonal
    Conjugate
    • 26
    • 9
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This WISP1 antibody is un-conjugated
    Application
    • 35
    • 21
    • 19
    • 13
    • 13
    • 8
    • 5
    • 5
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    WISP1
    Sequence
    ESYPDFSEIA N
    Specificity
    This antibody is expected to recognize both reported isoforms (NP_003873.1, NP_543028.1).
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-ESYPDFSEIAN, from the C Terminus of the protein sequence according to NP_003873.1, NP_543028.1.
    Isotype
    IgG
  • Application Notes

    Western Blot: Approx 32-35 kDa band observed in lysates of cell line CaCo-2 and approx. 40 kDa in Human Tonsil lysates, and additionally in a lysate of cell line CaCo-2, which were successfully blocked by incubation with the immunising peptide (calculated

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
    Alternative Name
    CCN4 (WISP1 Products)
    Synonyms
    CCN4 antibody, WISP1c antibody, WISP1i antibody, WISP1tc antibody, WISP-1 antibody, WISP1 antibody, si:ch211-38f10.2 antibody, AW146261 antibody, Elm1 antibody, zgc:172276 antibody, WNT1 inducible signaling pathway protein 1 antibody, WNT1 inducible signaling pathway protein 1a antibody, WNT1-inducible-signaling pathway protein 1-like antibody, WNT1 inducible signaling pathway protein 1b antibody, WISP1 antibody, wisp1a antibody, Wisp1 antibody, LOC100627044 antibody, wisp1b antibody
    Background
    WISP1, WNT1 inducible signaling pathway protein 1, CCN4, WISP1c, WISP1i, WISP1tc, WNT1 induced secreted protein 1, Wnt-1 inducible signaling pathway protein 1, wnt-1 signaling pathway protein 1
    Gene ID
    8840, 22402, 65154
    NCBI Accession
    NP_003873, NP_543028
    Pathways
    WNT Signaling, Growth Factor Binding
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