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alpha-Mannosidase II antibody (Mannosidase, Alpha, Class 2A, Member 1) (Internal Region)

Details for Product anti-MAN2A1 Antibody No. ABIN238628, Supplier: Log in to see
Antigen
  • CG18474
  • CG18802
  • CG8139
  • DM-GII.1
  • Dmel\\CG18802
  • GMII
  • GmII
  • MAN-2
  • Man
  • dGMII
  • dGMIIb
  • MAN2A1
  • si:ch211-131c14.1
  • Mana-2
  • Mana2
  • Map-2
  • GOLIM7
  • MANA2
  • MANII
  • Manno
Epitope
Internal Region
18
17
9
3
2
1
1
Reactivity
Human
35
1
Host
Goat
24
7
4
Clonality
Polyclonal
Conjugate
This alpha-Mannosidase II antibody is un-conjugated
2
2
2
2
2
2
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
27
22
15
3
2
1
1
1
1
Supplier
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Immunogen Peptide with sequence C-KISSDIKSQNR, from the internal region of the protein sequence according to NP_002363.2.
Blocking Peptide Blocking peptide for this product available: ABIN369290
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name MAN2A1 (MAN2A1 Antibody Abstract)
Background Synonyms: MAN2A1, mannosidase, alpha, class 2A, member 1, GOLIM7, MANA2, MANII, Golgi alpha-mannosidase II, golgi integral membrane protein 7, mannosidase, alpha type II, mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
Gene ID 4124
NCBI Accession NP_002363
Pathways
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 150 kDa band observed in Human Placenta lysates (calculated MW of 131 kDa according to NP_002363.2). In transfected HEK293 transiently expressing MAN2A1 a band of approx. 98 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 µg/mL. An anonymous customer found positive results in WB on Human dermal fibroblasts.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-alpha-Mannosidase II antibody (Mannosidase, Alpha, Class 2A, Member 1) (Internal Region) (ABIN238628) ABIN238628 (0.3µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer)...
Background publications Green, Stone, Tenno et al.: "Mammalian N-glycan branching protects against innate immune self-recognition and inflammation in autoimmune disease pathogenesis." in: Immunity, Vol. 27, Issue 2, pp. 308-20, 2007 (PubMed).