Mannosidase, Alpha, Class 2A, Member 1 (MAN2A1) (Internal Region) antibody

Details for Product No. ABIN238628
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Synonyms MANA2, MANII, GOLIM7, Mana2, Manno, Mana-2, Map-2, CG18474, CG18802, CG8139, DM-GII.1, Dmel\\CG18802, GMII, GmII, MAN-2, Man, dGMII, dGMIIb, MAN2A1, si:ch211-131c14.1
Internal Region
(3), (2), (2), (1), (1), (1)
(16), (2)
(10), (4), (3)
Methode Type Peptide ELISA
ELISA, Western Blotting (WB)
(9), (6), (4), (3), (2), (2), (2)
Pubmed 1 reference available
Catalog no. ABIN238628
Quantity 100 µg
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-KISSDIKSQNR, from the internal region of the protein sequence according to NP_002363.2.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name MAN2A1
Background Synonyms: MAN2A1, mannosidase, alpha, class 2A, member 1, GOLIM7, MANA2, MANII, Golgi alpha-mannosidase II, golgi integral membrane protein 7, mannosidase, alpha type II, mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
Gene ID 4124
NCBI Accession NP_002363.2
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 150 kDa band observed in Human Placenta lysates (calculated MW of 131 kDa according to NP_002363.2). In transfected HEK293 transiently expressing MAN2A1 a band of approx. 98 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 µg/mL. An anonymous customer found positive results in WB on Human dermal fibroblasts.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Mannosidase, Alpha, Class 2A, Member 1 (MAN2A1) (Internal Region) antibody ABIN238628 (0.3µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Green, Stone, Tenno et al.: "Mammalian N-glycan branching protects against innate immune self-recognition and inflammation in autoimmune disease pathogenesis." in: Immunity, Vol. 27, Issue 2, pp. 308-20, 2007 (PubMed).

Hosts (10), (4), (3)
Reactivities (16), (2)
Applications (9), (6), (4), (3), (2), (2), (2)
Epitopes (3), (2), (2), (1), (1), (1)
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