Annexin V antibody (Biotin)

Catalog No. ABIN576509

Product Details anti-Annexin V Antibody (Biotin)

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Annexin V antibody is conjugated to Biotin

Application: ELISA, Flow Cytometry (FACS)

Specificity: Recognizes human Annexin V, a phospholipid binding protein which binds with high affinity and selectivity to phosphatidylserine (PS), in the presence of calcium. The translocation of PS from the cytosolic leaflet to the outer leaflet of the plasma membrane, due to a loss in plasma membrane asymmetry during apoptosis, acts as an in vivo signal for the removal of the dying cell by phagocytosis. Detection of PS using Annexin V therefore provides a reliable diagnostic tool for the indication of early programmed cell death. Annexin V has also been identified as an anticoagulant protein in the blood coagulation cascade, by acting as an inhibitor of prothrombin activation. The presence of antibodies to Annexin V, are associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc). Clone VAA-33 has been reported to work in western blotting applications.

Purification: Protein G purified

Immunogen: Recombinant Annexin V.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, Flo

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Method sheets are available on request.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline, 1 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Annexin V

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Alternative Name: ANXA5 / Annexin V (ANXA5 Products)

Background: Name/Gene ID: ANXA5

Synonyms: ANXA5, ANX5, Anchorin CII, Calphobindin I, CBP-I, ENX2, PP4, RPRGL3, Lipocortin V, Thromboplastin inhibitor, VAC-alpha, Vascular anticoagulant-alpha, PAP-I, Annexin A5, Annexin V, Annexin-5, Endonexin II

Gene ID: 308

UniProt: P08758

Pathways: Apoptosis