XTH-Xet antibody

Details for Product No. ABIN249338, Supplier: Log in to see
Antigen
Reactivity
Barley (Hordeum vulgare), Rice (Oryza sativa)
Host
Rabbit
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB)
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Immunogen two synthetic peptides from highly conserved region of Horderum vulgare XTH-Xet
Cross-Reactivity (Details) Not reactive in: Zea mays
Predicted Reactivity monocots
Characteristics Expected / apparent Molecular Weight of the Antigene: 31.5 / 33 kDa
Purification serum
Background Molecular interactions between wall polysaccharides, which include cellulose and a range of non-cellulosic polysaccharides such as xyloglucans and (1,3,1,4)-β-d-glucans, are fundamental to cell wall properties. These interactions have been assumed to be non-covalent in nature in most cases. A highly purified barley xyloglucan xyloglucosyl transferase HvXET5 (EC 2.4.1.207), a member of the GH16 group of glycoside hydrolases, catalyses the in vitro formation of covalent linkages between xyloglucans and cellulosic substrates, and between xyloglucans and (1,3,1,4)-β-d-glucans. It is possible that XETs could link different polysaccharides in vivo, and hence influence cell wall strength, flexibility and porosity.
Molecular Weight expected: 31.5 kDa, apparent: 33 kDa
Application Notes 1: 500 with standard ECL (WB) , 1: 5000 (ELISA)
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 100 μL of sterile water.
Handling Advice Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Storage -20 °C
Storage Comment store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Product cited in: Hrmova, Farkas, Lahnstein, Fincher: "A Barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates, and (1,3;1,4)-beta-D-glucans." in: The Journal of biological chemistry, Vol. 282, Issue 17, pp. 12951-62, 2007 (PubMed).