XTH-Xet antibody
Reactivity: Barley, Oryza sativa
ELISA, WB
Host: Rabbit
Polyclonal
unconjugated
(1 reference)-
- Target
- XTH-Xet
- Reactivity
- Barley, Oryza sativa
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- ELISA, Western Blotting (WB)
- Cross-Reactivity (Details)
- Not reactive in: Zea mays
- Predicted Reactivity
- monocots
- Characteristics
- Expected / apparent Molecular Weight of the Antigene: 31.5 / 33 kDa
- Purification
- serum
- Immunogen
- two synthetic peptides from highly conserved region of Horderum vulgare XTH-Xet
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- Application Notes
- 1: 500 with standard ECL (WB) , 1: 5000 (ELISA)
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 100 μL of sterile water.
- Handling Advice
-
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - Storage
- -20 °C
- Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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: "A Barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates, and (1,3;1,4)-beta-D-glucans." in: The Journal of biological chemistry, Vol. 282, Issue 17, pp. 12951-62, (2007) (PubMed).
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: "A Barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates, and (1,3;1,4)-beta-D-glucans." in: The Journal of biological chemistry, Vol. 282, Issue 17, pp. 12951-62, (2007) (PubMed).
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- Target
- XTH-Xet
- Background
- Molecular interactions between wall polysaccharides, which include cellulose and a range of non-cellulosic polysaccharides such as xyloglucans and (1,3,1,4)-β-d-glucans, are fundamental to cell wall properties. These interactions have been assumed to be non-covalent in nature in most cases. A highly purified barley xyloglucan xyloglucosyl transferase HvXET5 (EC 2.4.1.207), a member of the GH16 group of glycoside hydrolases, catalyses the in vitro formation of covalent linkages between xyloglucans and cellulosic substrates, and between xyloglucans and (1,3,1,4)-β-d-glucans. It is possible that XETs could link different polysaccharides in vivo, and hence influence cell wall strength, flexibility and porosity.
- Molecular Weight
- expected: 31.5 kDa, apparent: 33 kDa
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