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Thylakoid Membrane Formation 1 (THF1) antibody

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Arabidopsis thaliana, Barley (Hordeum vulgare)
Immunoprecipitation (IP), Western Blotting (WB)
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Immunogen KLH-conjugated peptide derived from known sequences of Psb29 including Arabidopsis thaliana Q9SKT0, At2g20890
Characteristics Expected / apparent Molecular Weight of the Antigene: 33.7 / 22 kDa
Purification serum
Alternative Name Thylakoid membrane formation 1, THF1 (Psb29)
Background AGI Code: At2g20890
Psb29 (THF1) is a conserved 22 kDa protein which functions in biogenesis of photosystem II complexes. This protein is required for organization of vesicles into mature thylakoid stacks for chloroplast development. Mediates G-protein signalling between the plasma membrane and the plastid.
Molecular Weight expected: 33.7 kDa, apparent: 22 kDa
UniProt Q9SKT0
Application Notes Recommended Dilution: 1 : 10 000 with standard ECL (WB), 5 µg in 300 µl (IP).
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 100 µL of sterile water.
Buffer PBS pH 7.4
Handling Advice Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Storage -20 °C
Background publications Huang, Chen, Zhu, Hu, Zhang, Ma, He, Huang: "Arabidopsis thylakoid formation 1 is a critical regulator for dynamics of PSII-LHCII complexes in leaf senescence and excess light." in: Molecular plant, Vol. 6, Issue 5, pp. 1673-91, 2013 (PubMed).

Zhang, Paakkarinen, van Wijk, Aro: "Co-translational assembly of the D1 protein into photosystem II." in: The Journal of biological chemistry, Vol. 274, Issue 23, pp. 16062-7, 1999 (PubMed).

Laemmli: "Cleavage of structural proteins during the assembly of the head of bacteriophage T4." in: Nature, Vol. 227, Issue 5259, pp. 680-5, 1970 (PubMed).