CD45RC antibody

Catalog No. ABIN292034

Product Details anti-CD45RC Antibody

Target: CD45RC

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45RC antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes rat CD45RC, the high molecular weight form of the leucocyte common antigen. The antigen is found on B cells, approximately 50 % of bone marrow cells, all CD8+ve T cells, but splits CD4+ve T cells into two populations, CD4+CD45RChigh (Th1-like) and CD4+CD45RClow (Th2-like). This product is routinely tested in flow cytometry on rat splenocytes.

Purification: Affinity purified

Endotoxin Level: Low Endotoxin

Immunogen: PHA stimulated rat lymphocytes.

Type of Immunogen: Cells

Clone: OX-22

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1:100 - 1:200), IHC, IHC-Fr, IHC-P

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request. Immunohistology: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4.

Storage: -20 °C

Storage Comment: Short term: -20°C
Long term: -20°C.

Target Details for CD45RC

Target: CD45RC

Alternative Name: CD45RC (CD45RC Products)