V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) (C-Term) antibody

Details for Product No. ABIN302092
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Synonyms LOC100136746, RNCMYC, c-myc, mMyc, AU016757, Myc2, Niard, Nird, bHLHe39, MYC, cmyc, zc-myc, MRTL, MYCC, c-Myc, C-MYC, CMYC, CMyc
(55), (51), (47), (35), (34), (17), (15), (14), (14), (11), (7), (5), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Human, All Species
(501), (181), (153), (45), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
(356), (175), (25), (14), (4), (3)
Clonality (Clone)
Monoclonal ()
(34), (32), (24), (8), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1)
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Flow Cytometry (FACS), Western Blotting (WB)
(411), (174), (165), (110), (109), (106), (51), (40), (40), (30), (27), (18), (15), (10), (8), (6), (3), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN302092
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Immunogen Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc.
Clone 9-00E-10
Isotype IgG1
Specificity The antibody 9E10 may be used to detect the c-Myc tag. The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells.
Purification Purified by protein A
Purity > 95 % (by SDS-PAGE)
Alternative Name c-Myc
Target Type Tag
Research Area Cancer, Cell Cycle, Transcription Factors
Application Notes Flow Cytometry: Recommended dilution: 1-5 µg/mL
Application note: Membrane permeabilization is required.
Immunoprecipitation: Recommended dilution: 1-5 µg/mL
Application note: not suitable for immunoprecipitation of native c-Myc protein
Western Blotting: Recommended dilution: 0,5-2 µg/mL
Positive control: c-Myc tagged proteinImmunohistochemistry (paraffin sections) Recommended dilution: 5-10 µg/mL
Positive tissue: perfused brain sections, liver, spleen

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
anti-V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) (C-Term) antibody Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
General Veracini, Simon, Richard et al.: "The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1." in: The Journal of cell biology, Vol. 182, Issue 3, pp. 603-14, 2008 (PubMed).

Wang, Campoli, Ko et al.: "Enhancement of scFv fragment reactivity with target antigens in binding assays following mixing with anti-tag monoclonal antibodies." in: Journal of immunological methods, Vol. 294, Issue 1-2, pp. 23-35, 2004 (PubMed).

Fujiwara, Poikonen, Aleman et al.: "A single-chain antibody/epitope system for functional analysis of protein-protein interactions." in: Biochemistry, Vol. 41, Issue 42, pp. 12729-38, 2002 (PubMed).

Baggio, Burgstaller, Hale et al.: "Identification of epitope-like consensus motifs using mRNA display." in: Journal of molecular recognition : JMR, Vol. 15, Issue 3, pp. 126-34, 2002 (PubMed).

Hilpert, Hansen, Wessner et al.: "Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose." in: Protein engineering, Vol. 14, Issue 10, pp. 803-6, 2001 (PubMed).

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