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Details for Product No. ABIN302092

V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) (C-Term) antibody

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Antigen
Synonyms MRTL, MYCC, bHLHe39, c-Myc, CMYC, c-myc, RNCMYC, mMyc, C-MYC, MYC, cmyc, zc-myc, AU016757, Myc2, Niard, Nird, LOC100136746
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Human, All Species
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Pubmed 5 references available
Catalog no. ABIN302092
Quantity 0.1 mg
Price
319.44 $   Plus shipping costs $45.00
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Availability Will be delivered in 4 to 5 Business Days
Immunogen Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region ofhuman c-Myc.
Clone 9E10
Isotype IgG1
Specificity The antibody 9E10 may be used to detect the c-Myc tag.The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-mycgene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is atranscription factor (nuclear localization). c-Myc is commonly activated in a variety of tumorcells and plays an important role in cellular proliferation, differentiation, apoptosis and cellcycle progression. The phosphorylation of c-Myc has been investigated and previousstudies have suggested a functional association between phosphorylation at Thr58/Ser62 byglycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase(JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc istightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is alsoessential for tumor cell development in vasculogenesis and angiogenesis that distributeblood throughout the cells.
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Alternative Name c-Myc
Research Area Cancer, Cell Cycle, Transcription Factors
Application Notes Flow Cytometry: Recommended dilution: 1-5 µg/ml. Application note: Membrane permeabilization is required. Immunoprecipitation - Recommended dilution: 1-5 µg/ml. Application note: not suitable for immunoprecipitation of native c-Myc proteinWestern Blotting - Recommended dilution: 0,5-2 µg/ml. Positive control: c-Myc tagged proteinImmunohistochemistry (paraffin sections) - Recommended dilution: 5-10 µg/ml. Positive tissue: perfused brain sections, liver, spleen
Restrictions For Research Use only
Concentration 1 mg/ml
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Storage 4 °C
Storage Comment Do not freeze. Do not use after expiration date stamped on vial label.
Hilpert, Hansen, Wessner et al.: "Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose." in: Protein engineering, Vol. 14, Issue 10, pp. 803-6, 2001 (PubMed).

Baggio, Burgstaller, Hale et al.: "Identification of epitope-like consensus motifs using mRNA display." in: Journal of molecular recognition : JMR, Vol. 15, Issue 3, pp. 126-34, 2002 (PubMed).

Fujiwara, Poikonen, Aleman et al.: "A single-chain antibody/epitope system for functional analysis of protein-protein interactions." in: Biochemistry, Vol. 41, Issue 42, pp. 12729-38, 2002 (PubMed).

Wang, Campoli, Ko et al.: "Enhancement of scFv fragment reactivity with target antigens in binding assays following mixing with anti-tag monoclonal antibodies." in: Journal of immunological methods, Vol. 294, Issue 1-2, pp. 23-35, 2004 (PubMed).

Veracini, Simon, Richard et al.: "The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1." in: The Journal of cell biology, Vol. 182, Issue 3, pp. 603-14, 2008 (PubMed).

Alternatives for antigen "V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC)", type "Antibodies"
Hosts (295), (155), (19), (17), (4), (4)
Reactivities (436), (196), (168), (28), (26), (25), (25), (20), (13), (7), (6), (5), (5), (5), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applications (345), (155), (126), (123), (113), (85), (50), (50), (41), (21), (10), (8), (7), (7), (3), (1), (1)
Conjugates (29), (24), (18), (7), (5), (5), (5), (5), (5), (5), (5), (5), (2), (1), (1), (1), (1), (1)
Epitopes (43), (40), (33), (32), (25), (18), (11), (10), (9), (6), (5), (5), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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