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C-MYC antibody (V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian)) (C-Term)

Details for Product anti-MYC Antibody No. ABIN302092, Supplier: Log in to see
Antigen
  • LOC100136746
  • RNCMYC
  • c-myc
  • mMyc
  • AU016757
  • Myc2
  • Niard
  • Nird
  • bHLHe39
  • MYC
  • cmyc
  • zc-myc
  • MRTL
  • MYCC
  • c-Myc
  • C-MYC
  • CMYC
  • CMyc
Alternatives
anti-Human C-MYC antibody for Flow Cytometry
Epitope
C-Term
98
67
60
40
37
35
27
25
21
18
17
15
13
13
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, All Species
657
204
164
14
8
7
7
6
5
5
5
5
4
3
2
1
1
1
1
1
1
Host
Mouse
447
199
16
13
8
1
Clonality (Clone)
Monoclonal ()
Conjugate
This C-MYC antibody is un-conjugated
40
32
28
15
14
12
5
4
4
4
4
4
4
4
4
3
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
443
242
213
118
108
92
69
45
42
36
22
14
11
10
7
6
4
4
2
2
2
Supplier
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Immunogen Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc.
Clone 9-00E-10
Isotype IgG1
Specificity The antibody 9E10 may be used to detect the c-Myc tag. The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells.
Purification Purified by protein A
Purity > 95 % (by SDS-PAGE)
Alternative Name c-Myc (MYC Antibody Abstract)
Target Type Tag
Research Area Cancer, Cell Cycle, Transcription Factors
Pathways p53 Signaling, Cell Division Cycle
Application Notes Flow Cytometry: Recommended dilution: 1-5 µg/mL
Application note: Membrane permeabilization is required.
Immunoprecipitation: Recommended dilution: 1-5 µg/mL
Application note: not suitable for immunoprecipitation of native c-Myc protein
Western Blotting: Recommended dilution: 0,5-2 µg/mL
Positive control: c-Myc tagged proteinImmunohistochemistry (paraffin sections) Recommended dilution: 5-10 µg/mL
Positive tissue: perfused brain sections, liver, spleen

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
 image for anti-C-MYC antibody (V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian)) (C-Term) (ABIN302092) Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Product cited in: Jelínková, Šafaříková, Vondálová Blanářová et al.: "Platinum(IV) complex LA-12 exerts higher ability than cisplatin to enhance TRAIL-induced cancer cell apoptosis via stimulation of mitochondrial pathway." in: Biochemical pharmacology, Vol. 92, Issue 3, pp. 415-24, 2014 (PubMed).

Veracini, Simon, Richard et al.: "The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1." in: The Journal of cell biology, Vol. 182, Issue 3, pp. 603-14, 2008 (PubMed).

Wang, Campoli, Ko et al.: "Enhancement of scFv fragment reactivity with target antigens in binding assays following mixing with anti-tag monoclonal antibodies." in: Journal of immunological methods, Vol. 294, Issue 1-2, pp. 23-35, 2004 (PubMed).

Fujiwara, Poikonen, Aleman et al.: "A single-chain antibody/epitope system for functional analysis of protein-protein interactions." in: Biochemistry, Vol. 41, Issue 42, pp. 12729-38, 2002 (PubMed).

Baggio, Burgstaller, Hale et al.: "Identification of epitope-like consensus motifs using mRNA display." in: Journal of molecular recognition : JMR, Vol. 15, Issue 3, pp. 126-34, 2002 (PubMed).

Cermák, Símová, Pintzas et al.: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." in: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, 2002 (PubMed).

Hilpert, Hansen, Wessner et al.: "Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose." in: Protein engineering, Vol. 14, Issue 10, pp. 803-6, 2001 (PubMed).

Siegel, Brandner, Hess: "Cross-reactivity of the monoclonal antibody 9E10 with murine c-MYC." in: International journal of oncology, Vol. 13, Issue 6, pp. 1259-62, 1999 (PubMed).

Background publications Hoffman, Amanullah, Shafarenko et al.: "The proto-oncogene c-myc in hematopoietic development and leukemogenesis." in: Oncogene, Vol. 21, Issue 21, pp. 3414-21, 2002 (PubMed).

Boxer, Dang: "Translocations involving c-myc and c-myc function." in: Oncogene, Vol. 20, Issue 40, pp. 5595-610, 2001 (PubMed).

Dang, Resar, Emison et al.: "Function of the c-Myc oncogenic transcription factor." in: Experimental cell research, Vol. 253, Issue 1, pp. 63-77, 1999 (PubMed).

Prendergast: "Mechanisms of apoptosis by c-Myc." in: Oncogene, Vol. 18, Issue 19, pp. 2967-87, 1999 (PubMed).

Evan, Lewis, Ramsay et al.: "Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product." in: Molecular and cellular biology, Vol. 5, Issue 12, pp. 3610-6, 1986 (PubMed).

Spandidos, Pintzas, Kakkanas et al.: "Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue." in: Anticancer research, Vol. 7, Issue 6, pp. 1299-304, 1988 (PubMed).