Apolipoprotein L, 1 (APOL1) (C-Term) antibody

Details for Product No. ABIN308434
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Antigen
Synonyms wu:fa95b12, wu:fc30h04, si:rp71-1g18.12, APO-L, APOL, APOL-I, FSGS4
Epitope
C-Term
(9), (6), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(59)
Host
Goat
(45), (8), (7)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(51), (29), (13), (10), (6), (3), (3), (2)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN308434
374.27 $
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Immunogen Peptide with sequence C-NNNYKILQADQE, from the C Terminus of the protein sequence according to NP_003652.2, NP_663318.1.
Specificity This antibody is expected to recognize both reported isoforms (NP_003652.2, NP_663318.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name APOL1
Background Synonyms: APOL1, apolipoprotein L, 1, APO-L, APOL, APOL-I, OTTHUMP00000028705, apolipoprotein L-I, apolipoprotein L1
Gene ID 8542
NCBI Accession NP_003652, NP_663318
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 45 kDa band observed in Human Placenta lysates (calculated MW of 44 kDa according to NP_003652.2 and of 45.9 kDa according to NP_663318.1 ). Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Apolipoprotein L, 1 (APOL1) (C-Term) antibody ABIN308434 (1µg/ml) staining of Human Frontal Cortex lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Pérez-Morga, Vanhollebeke, Paturiaux-Hanocq et al.: "Apolipoprotein L-I promotes trypanosome lysis by forming pores in lysosomal membranes." in: Science (New York, N.Y.), Vol. 309, Issue 5733, pp. 469-72, 2005 (PubMed).

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