Dihydrolipoyl Transacetylase (DLAT) (N-Term) antibody

Details for Product No. ABIN310264
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Antigen
Synonyms DLAT, Dmel\\CG5261, EP(2)0816, EP816, anon-WO0118547.121, DLTA, PDC-E2, PDCE2, wu:fc14f10, wu:fc21f08, wu:fc86g11, wu:fj57d06, 6332404G05Rik
Epitope
N-Term
(12), (7), (2), (2), (2), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine), Pig (Porcine), Xenopus laevis, Zebrafish (Danio rerio), Human, Chicken
(27), (10), (8), (3), (3), (2), (1), (1), (1), (1)
Host
Rabbit
(23), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(28), (12), (11), (9), (5), (4), (2), (2), (2), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN310264
229.00 $
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Immunogen Synthetic peptide directed towards the N terminal of human DLAT
Sequence WTPSSGATPR NRLLLQLLGS PGRRYYSLPP HQKVPLPSLS PTMQAGTIAR
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Pig : 100 %, Rabbit : 100 %, African clawed frog : 90 %, Zebrafish : 80 %, Chicken : 70 %
Characteristics This is a rabbit polyclonal antibody against DLAT. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Alternative Name DLAT
Background DLAT is dihydrolipoamide acetyltransferase, the E2 subunit of the mammalian pyruvate dehydrogenase complex of the inner mitochondrial membrane. Patients with primary biliary cirrhosis show autoantibodies to DLAT.The DLAT gene encodes dihydrolipoamide acetyltransferase (EC 2.3.1.12), the E2 subunit of the mammalian pyruvate dehydrogenase complex (PDC, EC 1.2.4.1) of the inner mitochondrial membrane. Patients with primary biliary cirrhosis (PBC, MIM 109720) show autoantibodies to DLAT.
Molecular Weight 69 kDa
Gene ID 1737
NCBI Accession NP_001922, NM_001931
UniProt P10515
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 647 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Dihydrolipoyl Transacetylase (DLAT) (N-Term) antibody WB Suggested Anti-DLAT Antibody Titration: 5.0ug/ml
Positive Control: Jurkat cell lysate
anti-Dihydrolipoyl Transacetylase (DLAT) (N-Term) antibody (2) Sample Type: Huh7 lysate (50ug)
Primary Antibody Dilution: 1:200
Image Submitted By: Partha Kasturi
University of Kansas Medical Center
anti-Dihydrolipoyl Transacetylase (DLAT) (N-Term) antibody (3) anti-Dihydrolipoyl Transacetylase (DLAT) (N-Term) antibody (Image 3)
anti-Dihydrolipoyl Transacetylase (DLAT) (N-Term) antibody (4) WB Suggested Anti-DLAT
Antibody Titration: 5.0 µg/mL
Positive Control: Jurkat cell lysate
Background publications Head, Brown, Zolkipli et al.: "Clinical and genetic spectrum of pyruvate dehydrogenase deficiency: dihydrolipoamide acetyltransferase (E2) deficiency." in: Annals of neurology, Vol. 58, Issue 2, pp. 234-41, 2005 (PubMed).

Validation Images
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