Dolichyl-diphosphooligosaccharide--Protein Glycosyltransferase (DDOST) (N-Term) antibody

Details for Product No. ABIN310826
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Antigen
Synonyms CG9022, DmOST50, Dmel\\CG9022, DrOST, OST48, OST48/WBP1, Ots48, anon-EST:Liang-1.11, clone 1.11, ost48, DDOST, AGER1, CDG1R, OKSWcl45, OST, WBP1, wu:fa11d01, zgc:66068, zgc:77478, Ddost
Epitope
N-Term
(6), (2), (2), (2), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine), Xenopus laevis, Pig (Porcine), Human, Zebrafish (Danio rerio), Chicken
(30), (7), (3), (1), (1)
Host
Rabbit
(29), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (3), (3)
Application
Western Blotting (WB)
(30), (30), (15), (6), (4), (4), (2), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN310826
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Immunogen Synthetic peptide directed towards the N terminal of human DDOST
Sequence SPSVEDFGGN INVETISAFI DGGGSVLVAA SSDIGDPLRE LGSECGIEFD
Predicted Reactivity Bovine : 100 %, Chicken : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, Zebrafish : 100 %
Characteristics This is a rabbit polyclonal antibody against DDOST. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Alternative Name DDOST
Background DDOST is a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. DDOST is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.
Molecular Weight 46 kDa
Gene ID 1650
NCBI Accession NP_005207, NM_005216
UniProt Q5VWA5
Research Area Organelles
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 456 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Dolichyl-diphosphooligosaccharide--Protein Glycosyltransferase (DDOST) (N-Term) antibody WB Suggested Anti-DDOST Antibody Titration: 5.0ug/ml
Positive Control: Jurkat cell lysate
Background publications Shibatani, David, McCormack et al.: "Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits." in: Biochemistry, Vol. 44, Issue 16, pp. 5982-92, 2005 (PubMed).

Validation Images
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