Coatomer Protein Complex, Subunit beta 1 (COPB1) (Middle Region) antibody

Details for Product No. ABIN311506
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Synonyms id:ibd5034, wu:fc66c05, zgc:92482, COPB1, DKFZp459K154, COPB, 2610019B04Rik, Rack2, Beta-COP
Middle Region
(21), (15), (3), (2), (1), (1), (1), (1), (1)
Mouse (Murine), Rat (Rattus), Cow (Bovine), Dog (Canine), Pig (Porcine), Xenopus laevis, Zebrafish (Danio rerio), Fruit Fly (Drosophila melanogaster), Human, Chicken
(33), (3), (2), (1)
(28), (7)
(3), (3), (3), (3), (3), (3)
Western Blotting (WB)
(30), (19), (6), (4), (4), (2), (1), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN311506
289.00 $
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Immunogen Synthetic peptide directed towards the middle region of human COPB1
Predicted Reactivity Dog : 100 %, Guinea pig : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, African clawed frog : 90 %, Horse : 90 %
Characteristics This is a rabbit polyclonal antibody against COPB1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name COPB1
Background The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins, the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
Molecular Weight 107 kDa
Gene ID 1315
NCBI Accession NM_016451, NP_057535
UniProt P53618
Application Notes Optimal working dilutions should be determined experimentally by the investigator.

Antigen size: 953 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Coatomer Protein Complex, Subunit beta 1 (COPB1) (Middle Region) antibody WB Suggested Anti-COPB1 Antibody Titration: 0.2-1 ug/ml
Positive Control: Human Liver
Product cited in: Goryachev, Pokhilko: "Computational model explains high activity and rapid cycling of Rho GTPases within protein complexes." in: PLoS computational biology, Vol. 2, Issue 12, pp. e172, 2007 (PubMed).

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