Ubiquitin-Conjugating Enzyme E2F (Putative) (UBE2F) (N-Term) antibody

Details for Product No. ABIN311523
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Antigen
Synonyms NCE2, 2510010F15Rik, AI851109, RGD1307608, zgc:77005
Epitope
N-Term
(7), (3), (2), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Cow (Bovine), Dog (Canine), Xenopus laevis, Human
(38), (21), (20), (16), (16), (14), (12)
Host
Rabbit
(27), (11)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(26), (15), (10), (10), (2), (1), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN311523
289.00 $
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Immunogen Synthetic peptide directed towards the N terminal of human UBE2F
Sequence MLTLASKLKR DDGLKGSRTA ATASDSTRRV SVRDKLLVKE VAELEANLPC
Predicted Reactivity Bovine : 100 %, Human : 100 %, Rat : 100 %, African clawed frog : 90 %, Dog : 90 %
Characteristics This is a rabbit polyclonal antibody against UBE2F. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name UBE2F
Background UBE2F belongs to the ubiquitin-conjugating enzyme family. UBE2F accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins.
Molecular Weight 21 kDa
Gene ID 140739
NCBI Accession NP_542409, NM_080678
UniProt Q969M7
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 185 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Ubiquitin-Conjugating Enzyme E2F (Putative) (UBE2F) (N-Term) antibody WB Suggested Anti-UBE2F Antibody Titration: 0.2-1 ug/ml
Positive Control: Hela cell lysate
anti-Ubiquitin-Conjugating Enzyme E2F (Putative) (UBE2F) (N-Term) antibody (2) Sample Type: 1. Human NT-2 cells (60ug)
Primary Antibody Dilution: 2ug/ml
Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience
Secondary Antibody Dilution: 1: 20,000
Image Submitted by: Yuzhi Chen
University of Arkansas for Medical Science
Background publications Hillier, Graves, Fulton et al.: "Generation and annotation of the DNA sequences of human chromosomes 2 and 4. ..." in: Nature, Vol. 434, Issue 7034, pp. 724-31, 2005 (PubMed).

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