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Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) antibody (Biotin)

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  • CD32
  • CD32a
  • CD32A
  • CDw32
  • FCG2
  • FcGR
  • FCGR2
  • FCGR2A1
  • IGFR2
Dog (Canine), Human, Monkey
Clonality (Clone)
Monoclonal ()
Flow Cytometry (FACS)
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Immunogen K562 cell line. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 myeloma cell line.
Clone AT10
Isotype IgG1
Specificity This antibody recognizes the CD32 antigen. Clone AT10 blocks the binding of IgG to Fc gamma R11. SM1092LE is recommended for this purpose.
Purification Affinity Chromatography on Protein A
Alternative Name CD32 / FcRII-A (FCGR2A Antibody Abstract)
Background CD32 is a 40kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets.Synonyms: CDw32, FCG2, FCGR2A, FCGR2A1, Fc-gamma RII-a, Fc-gamma-RIIa, IGFR2, IgG Fc receptor II, Low affinity immunoglobulin gamma Fc region receptor II
Gene ID 2212
NCBI Accession NP_001129691
UniProt P12318
Application Notes Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4, 0.09 % Sodium Azide, 1 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Zhao, van Beek, Schornagel, Van der Maaden, Van Houdt, Otten, Finetti, Van Egmond, Matozaki, Kraal, Birnbaum, van Elsas, Kuijpers, Bertucci, van den Berg: "CD47-signal regulatory protein-α (SIRPα) interactions form a barrier for antibody-mediated tumor cell destruction." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 45, pp. 18342-7, 2011

Araújo, de Andrade, Sathler-Avelar, Magalhães, Carvalho, Andrade, Campolina, Mello, Vianna, Mayrink, Reis, Malaquias, Rocha, Martins-Filho: "Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis." in: Veterinary immunology and immunopathology, Vol. 141, Issue 1-2, pp. 64-75, 2011

Shannon, Mörgelin, Rasmussen: "Platelet activation and biofilm formation by Aerococcus urinae, an endocarditis-causing pathogen." in: Infection and immunity, Vol. 78, Issue 10, pp. 4268-75, 2010

Devriendt, Verdonck, Summerfield, Goddeeris, Cox: "Targeting of Escherichia coli F4 fimbriae to Fcgamma receptors enhances the maturation of porcine dendritic cells." in: Veterinary immunology and immunopathology, Vol. 135, Issue 3-4, pp. 188-98, 2010

Santer, Hall, George, Tangsombatvisit, Liu, Arkwright, Elkon: "C1q deficiency leads to the defective suppression of IFN-alpha in response to nucleoprotein containing immune complexes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 8, pp. 4738-49, 2010

Dutertre, Bonnin-Gélizé, Pulford, Bourel, Fridman, Teillaud: "A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody-dependent function." in: Journal of leukocyte biology, Vol. 84, Issue 6, pp. 1511-20, 2008

Khan, Kim, Kim: "Synthesis and control of physical properties of titania nanoparticles as a function of synthetic parameters." in: Journal of nanoscience and nanotechnology, Vol. 8, Issue 9, pp. 4738-42, 2008

Benitez-Ribas, Adema, Winkels, Klasen, Punt, Figdor, de Vries: "Plasmacytoid dendritic cells of melanoma patients present exogenous proteins to CD4+ T cells after Fc gamma RII-mediated uptake." in: The Journal of experimental medicine, Vol. 203, Issue 7, pp. 1629-35, 2006

Sims, Ettinger, Shirota, Yarboro, Illei, Lipsky: "Identification and characterization of circulating human transitional B cells." in: Blood, Vol. 105, Issue 11, pp. 4390-8, 2005

Lilliehöök, Johannisson, Håkansson: "Expression of adhesion and Fcgamma-receptors on canine blood eosinophils and neutrophils studied by anti-human monoclonal antibodies." in: Veterinary immunology and immunopathology, Vol. 61, Issue 2-4, pp. 181-93, 1998

Larsson, Berge, Johansson, Forsum: "Human dendritic cells handling of binding, uptake and degradation of free and IgG-immune complexed dinitrophenylated human serum albumin in vitro." in: Immunology, Vol. 90, Issue 1, pp. 138-46, 1997

Van Den Herik-Oudijk, Westerdaal, Henriquez, Capel, Van De Winkel: "Functional analysis of human Fc gamma RII (CD32) isoforms expressed in B lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 2, pp. 574-85, 1994

Greenman, Tutt, George, Pulford, Stevenson, Glennie: "Characterization of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors." in: Molecular immunology, Vol. 28, Issue 11, pp. 1243-54, 1992