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Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A) antibody

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Synonyms CD32, CD32A, CDw32, FCG2, FCGR2, FCGR2A1, FcGR, IGFR2, CD32a
Dog (Canine), Human, Monkey, Pig (Porcine)
(137), (7), (6), (4), (3), (2), (1)
(77), (70), (1)
Clonality (Clone)
Monoclonal ()
(11), (11), (9), (4), (4), (4)
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(100), (56), (40), (32), (29), (27), (17), (11), (4), (2), (2), (2), (1), (1), (1)
Pubmed 8 references available
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Quantity 25 μg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen K562 cell line. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Clone AT10
Isotype IgG1
Specificity This antibody recognises the CD32 antigen. Clone AT10 blocks the binding of IgG to Fc gamma R11. SM1092LE is recommended for this purpose.
Purification Affinity Chromatography on Protein A.
Alternative Name CD32 / FcRII-A (FCGR2A Antibody Abstract)
Background CD32 is a 40kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets.Synonyms: CDw32, FCG2, FCGR2A, FCGR2A1, Fc-gamma RII-a, Fc-gamma-RIIa, IGFR2, IgG Fc receptor II, Low affinity immunoglobulin gamma Fc region receptor II
Gene ID 2212
NCBI Accession NP_001129691
UniProt P12318
Application Notes Immunoprecipitation: 20 μg/mL. Flow Cytometry (20 μg/mL): Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood. Immunohistochemistry on Frozen Sections: 1/500-1/1000. Recommended Positive Control: Lymph node.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Devriendt, Verdonck, Summerfield et al.: "Targeting of Escherichia coli F4 fimbriae to Fcgamma receptors enhances the maturation of porcine dendritic cells." in: Veterinary immunology and immunopathology, Vol. 135, Issue 3-4, pp. 188-98, 2010 (PubMed).

Dutertre, Bonnin-Gélizé, Pulford et al.: "A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody-dependent function." in: Journal of leukocyte biology, Vol. 84, Issue 6, pp. 1511-20, 2008 (PubMed).

Benitez-Ribas, Adema, Winkels et al.: "Plasmacytoid dendritic cells of melanoma patients present exogenous proteins to CD4+ T cells after Fc gamma RII-mediated uptake." in: The Journal of experimental medicine, Vol. 203, Issue 7, pp. 1629-35, 2006 (PubMed).

Sims, Ettinger, Shirota et al.: "Identification and characterization of circulating human transitional B cells." in: Blood, Vol. 105, Issue 11, pp. 4390-8, 2005 (PubMed).

Lilliehöök, Johannisson, Håkansson: "Expression of adhesion and Fcgamma-receptors on canine blood eosinophils and neutrophils studied by anti-human monoclonal antibodies." in: Veterinary immunology and immunopathology, Vol. 61, Issue 2-4, pp. 181-93, 1998 (PubMed).

Larsson, Berge, Johansson et al.: "Human dendritic cells handling of binding, uptake and degradation of free and IgG-immune complexed dinitrophenylated human serum albumin in vitro." in: Immunology, Vol. 90, Issue 1, pp. 138-46, 1997 (PubMed).

Van Den Herik-Oudijk, Westerdaal, Henriquez et al.: "Functional analysis of human Fc gamma RII (CD32) isoforms expressed in B lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 2, pp. 574-85, 1994 (PubMed).

Greenman, Tutt, George et al.: "Characterization of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors." in: Molecular immunology, Vol. 28, Issue 11, pp. 1243-54, 1992 (PubMed).

Catalog No. ABIN319715
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