IL17D antibody (AA 25-205)

Catalog No. ABIN327476

Product Details anti-IL17D Antibody

Target: IL17D (Interleukin 17D (IL17D))

Binding Specificity: AA 25-205

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL17D antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Specificity: Recognizes mouse IL-17D. In direct ELISAs and Western Blots this antibody shows no cross-reactivity with rhIL-17D, rmIL-17, rmIL-17B, rmIL-17C, rmIL-17E or rmIL-17F.

Purification: Protein G purified

Immunogen: Recombinant protein corresponding to aa25-205 from mouse Interleukin 17D expressed in E. coli (NP_665836).

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, Flo, WB

Usage: Western Blot, Flow Cytometry, Direct ELISA. Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect mouse IL-17D. Using a colorimetric detection system, the detection limit for rmIL-17D is approximately 5 ng/lane under non-reducing and reducing conditions. Use of this antibody under non reducing/reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry: This antibody was validated for flow cytometry using CD4+ splenic lymphocytes. For intracellular staining to detect IL-17D, cells must first be fixed and permeabilized with 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse IL-17D. The detection limit for rmIL-17D is approximately 1 ng/well.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 200 μL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Storage: 4 °C,-20 °C

Storage Comment: Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.

Target Details for IL17D

Target: IL17D (Interleukin 17D (IL17D))

Alternative Name: IL17D (IL17D Products)

Background: Name/Gene ID: IL17D
Family: Interleukin

Synonyms: IL17D, IL-27, Interleukin 27, Interleukin-17D, Interleukin-27, IL-17D, IL-22, Interleukin 17D

Gene ID: 53342

NCBI Accession: NP_665836

UniProt: Q8TAD2

Pathways: Cellular Response to Molecule of Bacterial Origin