Acylglycerol Kinase (AGK) (C-Term) antibody

Details for Product No. ABIN334340
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Antigen
Synonyms mulk, CATC5, CTRCT38, MTDPS10, MULK, 2610037M15Rik, 6720408I04Rik, AI465370, Mulk, RGD1562046, fi38e09, wu:fi38e09, zgc:55462
Epitope
C-Term
(6), (2), (2), (2)
Reactivity
Human
(27), (12), (12), (12), (12), (12), (12)
Host
Goat
(21), (4), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(17), (10), (4), (4), (2), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN334340
374.27 $
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Immunogen Peptide with sequence CDPRKREQMLTSP, from the C Terminus of the protein sequence according to NP_060708.1.
Cross-Reactivity Mouse (Murine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Acylglycerol kinase
Background Synonyms: acylglycerol kinase, FLJ10842, MULK, multi-substrate lipid kinase, multiple substrate lipid kinase, AGK
Gene ID 55750, 69923
NCBI Accession NP_060708
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 50 kDa band observed in Human Brain (Substantia nigra, Hippocampus) lysates (calculated MW of 47.1 kDa according to NP_060708.1). Recommended concentration: 1-3 µg/mL. An additional band of unknown identity was also consistently observed at 22 kDa. This band was successfully blocked by incubation with the immunising peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Acylglycerol Kinase (AGK) (C-Term) antibody ABIN334340 (1µg/ml) staining of Human Brain (Substantia nigra) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Outeiro, Kontopoulos, Altmann et al.: "Sirtuin 2 inhibitors rescue alpha-synuclein-mediated toxicity in models of Parkinson's disease." in: Science (New York, N.Y.), Vol. 317, Issue 5837, pp. 516-9, 2007 (PubMed).

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