anti-BNIP1 antibody (BCL2/adenovirus E1B 19kDa Interacting Protein 1) (Internal Region)

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Antigen
Synonyms NIP1, SEC20, TRG-8, 2010005M06Rik, 5930429G21Rik, Sec20, 2900010M17Rik, AI853434, Apba2bp, Nip1, XB51
Epitope
Internal Region
(20), (16), (12), (11), (10), (4), (4), (4), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(109), (30), (27), (3), (1), (1), (1)
Host
Goat
(73), (23), (15)
Clonality
Polyclonal
Conjugate
This BNIP1 antibody is un-conjugated
(6), (6), (6), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(93), (50), (20), (11), (7), (4), (3), (1), (1), (1), (1)
Pubmed 1 reference available
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Quantity 100 μg
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Immunogen Peptide with sequence C-DLLRQRKTTKESLAQ, from the internal region of the protein sequence according to NP_001196.1, NP_053581.1, NP_053582.1, NP_053583.1.
Blocking Peptide Blocking peptide for this product available: BCL2/adenovirus E1B 19kDa Interacting Protein 1 (BNIP1) peptide (ABIN368890)
Specificity All reported variants represent isoform 1 (NP_001196.1, NP_053581.1, NP_053582.1, NP_053583.1)
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name BNIP1 (BNIP1 Antibody Abstract)
Background Synonyms: BNIP1, BCL2/adenovirus E1B 19kDa interacting protein 1, NIP1, SEC20, TRG-8, BCL2/adenovirus E1B 19kD interacting protein 1, BCL2/adenovirus E1B 19kD-interacting protein 1
Gene ID 662, 224630, 140932
NCBI Accession NP_053583, NP_053582, NP_053581, NP_001196
Research Area Signaling, Apoptosis/Necrosis
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 28 kDa band observed in Human Kidney lysates (calculated MW of 26.1 kDa according to NP_001196.1, NP_053581.1, NP_053582.1, NP_053583.1). Recommended concentration: 0.5-1.5 µg/mL. An additional band of unknown identity was also consistently observed at 48 kDa. This band was successfully blocked by incubation with the immunising peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Image no. 1 for anti-BNIP1 antibody (BCL2/adenovirus E1B 19kDa Interacting Protein 1) (Internal Region) (ABIN334362) ABIN334362 (0.5µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). ...
Background publications Nakajima, Hirose, Taniguchi et al.: "Involvement of BNIP1 in apoptosis and endoplasmic reticulum membrane fusion." in: The EMBO journal, Vol. 23, Issue 16, pp. 3216-26, 2004 (PubMed).

Catalog No. ABIN334362
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