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BCL2/adenovirus E1B 19kDa Interacting Protein 1 (BNIP1) (Internal Region) antibody

Details for Product No. ABIN334362
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Antigen
Synonyms NIP1, SEC20, TRG-8, 2010005M06Rik, 5930429G21Rik, Sec20, 2900010M17Rik, AI853434, Apba2bp, Nip1, XB51
Epitope
Internal Region
(9), (8), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(52), (19), (17), (12), (12), (12)
Host
Goat
(40), (9), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(43), (20), (10), (9), (5), (3), (2), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN334362
374.27 $
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Immunogen Peptide with sequence C-DLLRQRKTTKESLAQ, from the internal region of the protein sequence according to NP_001196.1, NP_053581.1, NP_053582.1, NP_053583.1.
Specificity All reported variants represent isoform 1 (NP_001196.1, NP_053581.1, NP_053582.1, NP_053583.1)
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name BNIP1
Background Synonyms: BNIP1, BCL2/adenovirus E1B 19kDa interacting protein 1, NIP1, SEC20, TRG-8, BCL2/adenovirus E1B 19kD interacting protein 1, BCL2/adenovirus E1B 19kD-interacting protein 1
Gene ID 662, 224630, 140932
NCBI Accession NP_001196.1, NP_053581.1, NP_053582.1, NP_053583.1
Research Area Signaling, Apoptosis/Necrosis
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 28 kDa band observed in Human Kidney lysates (calculated MW of 26.1 kDa according to NP_001196.1, NP_053581.1, NP_053582.1, NP_053583.1). Recommended concentration: 0.5-1.5 µg/mL. An additional band of unknown identity was also consistently observed at 48 kDa. This band was successfully blocked by incubation with the immunising peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-BCL2/adenovirus E1B 19kDa Interacting Protein 1 (BNIP1) (Internal Region) antibody ABIN334362 (0.5µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Nakajima, Hirose, Taniguchi et al.: "Involvement of BNIP1 in apoptosis and endoplasmic reticulum membrane fusion." in: The EMBO journal, Vol. 23, Issue 16, pp. 3216-26, 2004 (PubMed).

Validation Images
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