Estrogen-Related Receptor gamma (ESRRG) (Internal Region) antibody

Details for Product No. ABIN334393
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Antigen
Synonyms errb/g, esrrgl, errbeta/gamma, ESRRG, USH2A, err3, nr3b3, LOC100125509, LOC100219115, LOC100230542, errg2, ERR3, ERRgamma, NR3B3, Errg, mKIAA0832, errg, DKFZp459J0417, errgamma, esrrg
Epitope
Internal Region
(13), (5), (3), (3), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(57), (25), (23), (17), (15), (12), (2), (2), (1), (1), (1)
Host
Goat
(49), (6), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(38), (16), (10), (8), (3), (2), (2), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN334393
374.27 $
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Immunogen Peptide with sequence CRMSNKDRHIDS, from the internal region of the protein sequence according to NP_001429.2, NP_996317.1, NP_996318.1.
Specificity This antibody is expected to recognise the reported isoform NP_001429.2.
Cross-Reactivity Mouse (Murine), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ESRRG
Background Synonyms: ESRRG, DKFZp781L1617, Estrogen-related receptor gamma, ERR3, FLJ16023, KIAA0832, NR3B3
Gene ID 2104, 26381
NCBI Accession NP_001429, NP_996318, NP_996317
Research Area Hormones, Receptors
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 50 kDa band observed in Human Brain (Cerebellum) and Breast lysates (calculated MW of 51.3 kDa according to NP_NP_001429.2). In transfected HEK293 transiently expressing ESRRG a band of approx. 52 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.2-0.6 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Estrogen-Related Receptor gamma (ESRRG) (Internal Region) antibody ABIN334393 (0.2µg/ml) staining of Human Breast lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Dufour, Wilson, Huss et al.: "Genome-wide orchestration of cardiac functions by the orphan nuclear receptors ERRalpha and gamma." in: Cell metabolism, Vol. 5, Issue 5, pp. 345-56, 2007 (PubMed).

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