Fas Apoptotic Inhibitory Molecule (FAIM) (Internal Region) antibody

Details for Product No. ABIN334394
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Antigen
Synonyms FAIM, faim, FAIM1, EG623459
Epitope
Internal Region
(11), (8), (8), (2), (2), (1), (1)
Reactivity
Human
(47), (30), (22), (16), (12), (11), (11), (1), (1)
Host
Goat
(44), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(40), (21), (9), (8), (3)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN334394
374.27 $
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Immunogen Peptide with sequence C-KKYMEDRSKTTN, from the internal region of the protein sequence according to NP_001028202.1, NP_001028203.1, NP_001028204.1, NP_060617.1.
Specificity This antibody is expected to recognise all reported isoforms (NP_001028202.1, NP_001028203.1, NP_001028204.1, NP_060617.1).
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name FAIM1
Background Synonyms: FAIM1, FAIM, Fas apoptotic inhibitory molecule
Gene ID 55179, 23873, 140930
NCBI Accession NP_060617, NP_001028202, NP_001028203, NP_001028204
Research Area Apoptosis/Necrosis
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 25 kDa band observed in Human Lymph Node lysates (calculated MW of 24.0 kDa according to NP_001028202.1). Recommended concentration: 0.5-1 µg/mL.
IHC: In paraffin embedded Human Placenta shows cytoplasm staining of trophoblasts. Recommended concentration: 2-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Fas Apoptotic Inhibitory Molecule (FAIM) (Internal Region) antibody ABIN334394 (0.5µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Schneider, Fischer, Donohoe et al.: "A novel gene coding for a Fas apoptosis inhibitory molecule (FAIM) isolated from inducibly Fas-resistant B lymphocytes." in: The Journal of experimental medicine, Vol. 189, Issue 6, pp. 949-56, 1999 (PubMed).

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