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Ribosomal Protein L8 (RPL8) (Internal Region) antibody

Details for Product No. ABIN334478, Supplier: Login to see
Antigen
  • CG1263
  • Dmel\\CG1263
  • L8
  • M(3)62F
  • M(3)LS2
  • Rp L8
  • anon-EST:Posey5
  • rpL8
  • zgc:73105
  • zgc:77641
Epitope
Internal Region
19
12
8
2
1
1
1
1
1
Reactivity
Human
75
16
14
11
2
2
2
2
1
Host
Goat
55
19
1
Clonality
Polyclonal
Conjugate
Un-conjugated
8
8
8
3
3
3
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
66
53
28
10
2
2
2
Supplier
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Immunogen Peptide with sequence KAGRAYHKYKAKRNC, from the internal region of the protein sequence according to NP_000964.1, NP_150644.1.
Blocking Peptide Blocking peptide for this product available: ABIN370190
Specificity The reported variants represent identical protein (NP_000964.1 and NP_150644.1).
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ribosomal protein L8 (RPL8 Antibody Abstract)
Background Synonyms: ribosomal protein L8, 60S ribosomal protein L8
Gene ID 6132, 26961, 26962
NCBI Accession NP_150644, NP_000964
Pathways
Application Notes ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 28-30 kDa band observed in lysates of cell line HepG2 (calculated MW of 28.0 kDa according to NP_000964.1 and NP_150644.1). Recommended concentration: 0.01-0.03 µg/mL.
IHC: In paraffin embedded Human Cerebral Cortex shows cytoplasm staining, including axons, of stallate and pyramidal cells. Recommended concentration: 2-4 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Ribosomal Protein L8 (RPL8) (Internal Region) antibody (ABIN334478) ABIN334478 (0.01µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). P...
Background publications Swoboda, Somasundaram, Caputo et al.: "Shared MHC class II-dependent melanoma ribosomal protein L8 identified by phage display." in: Cancer research, Vol. 67, Issue 8, pp. 3555-9, 2007 (PubMed).