Ribosomal Protein L8 (RPL8) (Internal Region) antibody

Details for Product No. ABIN334478
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Antigen
Synonyms L8, zgc:73105, zgc:77641, CG1263, Dmel\\CG1263, M(3)62F, M(3)LS2, Rp L8, anon-EST:Posey5, rpL8
Epitope
Internal Region
(4), (4), (1), (1), (1)
Reactivity
Human
(22), (8), (8), (6), (2), (2), (2)
Host
Goat
(19), (6), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(25), (16), (6), (4), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN334478
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence KAGRAYHKYKAKRNC, from the internal region of the protein sequence according to NP_000964.1, NP_150644.1.
Specificity The reported variants represent identical protein (NP_000964.1 and NP_150644.1).
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ribosomal protein L8
Background Synonyms: ribosomal protein L8, 60S ribosomal protein L8
Gene ID 6132, 26961, 26962
NCBI Accession NP_000964.1, NP_150644.1
Application Notes ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 28-30 kDa band observed in lysates of cell line HepG2 (calculated MW of 28.0 kDa according to NP_000964.1 and NP_150644.1). Recommended concentration: 0.01-0.03 µg/mL.
IHC: In paraffin embedded Human Cerebral Cortex shows cytoplasm staining, including axons, of stallate and pyramidal cells. Recommended concentration: 2-4 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Ribosomal Protein L8 (RPL8) (Internal Region) antibody ABIN334478 (0.01µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Swoboda, Somasundaram, Caputo et al.: "Shared MHC class II-dependent melanoma ribosomal protein L8 identified by phage display." in: Cancer research, Vol. 67, Issue 8, pp. 3555-9, 2007 (PubMed).

Hosts (19), (6), (1)
Reactivities (22), (8), (8), (6), (2), (2), (2)
Applications (25), (16), (6), (4), (1), (1)
Conjugates (2), (2), (2)
Epitopes (4), (4), (1), (1), (1)
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