SAR1 Homolog B (S. Cerevisiae) (SAR1B) (Internal Region) antibody

Details for Product No. ABIN334486
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Antigen
Synonyms SAR1, SAR1B, SARA2, 2310075M17Rik, 2900019I22Rik, CMRD, Sara1b, Sara2, Sarb, cmrd, gtbpb, sar1a, sara2, SARA1B, ANDD, GTBPB, zgc:73204
Epitope
Internal Region
(13), (8), (2), (2), (2), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus)
(30), (16), (15), (1), (1), (1), (1), (1), (1)
Host
Goat
(27), (4), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(31), (20), (19), (9), (4), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN334486
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Immunogen Peptide with sequence C-DHERLLESKEELD, from the internal region of the protein sequence according to NP_001028675.1, NP_057187.1.
Specificity Both reported variants (NP_001028675.1 and NP_057187.1) represent identical protein
Cross-Reactivity Human
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SAR1B / SARA2
Background Synonyms: SAR1B, SARA2, SAR1 gene homolog B (S. cerevisiae), CMRD, GTBPB, 2310075M17Rik, GTP-binding protein Sara, SAR1a gene homolog 2
Gene ID 51128, 66397, 287276
NCBI Accession NP_057187, NP_001028675
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx. 25 kDa band observed in Mouse Liver and Rat Liver lysates (calculated MW of 22.4 kDa according to human NP_057187.1 and mouse NP_079811.1). In transfected HEK293 transiently expressing SAR1B a band of approx. 26 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-SAR1 Homolog B (S. Cerevisiae) (SAR1B) (Internal Region) antibody ABIN334486 (0.03µg/ml) staining of Mouse Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Evans, Burdge, Wootton et al.: "Tissue-specific stable isotope measurements of postprandial lipid metabolism in familial combined hyperlipidaemia." in: Atherosclerosis, Vol. 197, Issue 1, pp. 164-70, 2008 (PubMed).

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