Parkinson Protein 7 (PARK7) antibody

Details for Product No. ABIN335354
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Antigen
Synonyms dj1, zgc:103725, DJ-1, Dj1, DJ1, CAP1, SP22
Reactivity
Human, Mouse (Murine), Dog (Canine), Xenopus laevis
(183), (77), (62), (16), (15), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Host
Mouse
(152), (33), (9), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(10), (6), (6), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunoprecipitation (IP)
(172), (97), (49), (41), (26), (21), (12), (10), (8), (8), (8), (2), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN335354
351.27 $
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Immunogen 12B11F8 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with a synthetic peptide corresponding to amino acid residues 779-793 of human plakophilin-3 coupled to keyhole limpet hemocyanin. (KLHRDFRAKGYRKED).
Clone 12B11F8
Isotype IgG1
Specificity Human, mouse, dog and Xenopus laevis.
Purification Serum
Alternative Name PARK7 / DJ-1
Background Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells [1]. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin-3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalisation, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin-3 displays a dual intracellular localisation in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The human protein has a predicted molecular mass of 87 kD.
Application Notes 12B11F8 reacts with an epitope located between residues 779-793 in human plakophilin-3. 12B11F8 is suitable for immunocytochemistry (methanol-fixed cells), immunohistochemistry on frozen tissues, immunoprecipitation and Western blot detection. For optimal results, fixed cells or tissues should be treated with 0.2% Triton-X 100 for 5 min. prior to incubation with primary antibody. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
Restrictions For Research Use only
Storage 4 °C
General Bonnue, Gilbert, Hatzfeld et al.: "Defining desmosomal plakophilin-3 interactions." in: The Journal of cell biology, Vol. 161, Issue 2, pp. 403-16, 2003 (PubMed).

Bonné, van Hengel, Nollet et al.: "Plakophilin-3, a novel armadillo-like protein present in nuclei and desmosomes of epithelial cells." in: Journal of cell science, Vol. 112 ( Pt 14), pp. 2265-76, 1999 (PubMed).

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