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8-Hydroxyguanosine (8-OHDG) antibody

Details for Product No. ABIN335420, Supplier: Log in to see
Clonality (Clone)
Monoclonal ()
Immunohistochemistry (IHC), ELISA
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Immunogen 8-OHdG-conjugated Keyhole Limpet Hemocyanin
Clone N45-1
Isotype IgG1 kappa
Specificity 8-OHDG in all species. No observable crossreactivity for the following analogues: guanosine (G), 7methylG, 6SHG, 8bromoG, dA, dC, dT, dI, dU, dG, O6methyldG, 8OHdA, guanine (Gua), O6methylGua, 8OHGua, uric acid, Urea, creatine, creatinine Slight but minimal (<1 %) crossreactivity for the following analogues: 8sulfhydrylG and 8OHG 1
Purification Prepared as ascites then ammonium sulphate purified
Alternative Name 8 Hydroxyguanosine (8-OHDG)
Target Type Chemical
Background 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical, singlet oxygen and direct photodynamic action. Anti 8-OHdG monoclonal antibody (clone N45.1) is highly specific for 8-OHdG, and is suitable for immunohistochemistry. Cross reactivity have been checked for 8-OHdG analogues (guanosine(G),7-methyl-G, 6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG, 8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G).
Research Area DNA/RNA
Application Notes Tested for IHC and ELISA. User should determine optimum titer for each application.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized as 100 ug/mL IgG in10 mM Phosphate buffered saline, pH7.4 containing 1.0 % BSA.
Storage -20 °C
Supplier Images
 image for anti-8-Hydroxyguanosine (8-OHDG) antibody (ABIN335420) Epidermis from hairless mouse by chronic UVB irradiation after 4 weeks of treatment s...
Product cited in: Baila, Ohno, Nagamoto, Kotosai, Yabuuchi, Funaguchi, Ito, Endo, Mori, Takemura, Fujiwara, Fujiwara, Minatoguchi: "Tetomilast attenuates elastase-induced pulmonary emphysema through inhibition of oxidative stress in rabbits." in: Biological & pharmaceutical bulletin, Vol. 35, Issue 4, pp. 494-502, 2012 (PubMed).

Sintara, Thong-Ngam, Patumraj, Klaikeaw: "Curcumin attenuates gastric cancer induced by N-methyl-N-nitrosourea and saturated sodium chloride in rats." in: Journal of biomedicine & biotechnology, Vol. 2012, pp. 915380, 2012 (PubMed).

Toyokuni, Hirao, Wada, Nagai, Date, Yoshii, Akatsuka, Yamashita, Kawada: "Age- and sun exposure-dependent differences in 8-hydroxy-2'-deoxyguanosine and N-(carboxymethyl)lysine in human epidermis." in: Journal of clinical biochemistry and nutrition, Vol. 49, Issue 2, pp. 121-4, 2011 (PubMed).

Sun, Sugiyama, Masuda, Ochi, Takeuchi: "Expressions of protein oxidation markers, dityrosine and advanced oxidation protein products in acetaminophen-induced liver injury in rats." in: The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Vol. 73, Issue 9, pp. 1185-90, 2011 (PubMed).

Calveley, Jelveh, Langan, Mahmood, Yeung, Van Dyk, Hill: "Genistein can mitigate the effect of radiation on rat lung tissue." in: Radiation research, Vol. 173, Issue 5, pp. 602-11, 2010 (PubMed).

Rabbani, Mi, Zhang, Delong, Jackson, Fleckenstein, Salahuddin, Zhang, Clary, Anscher, Vujaskovic: "Hypoxia inducible factor 1alpha signaling in fractionated radiation-induced lung injury: role of oxidative stress and tissue hypoxia." in: Radiation research, Vol. 173, Issue 2, pp. 165-74, 2010 (PubMed).

Mori, Nakamachi, Ohtaki, Yofu, Sato, Endo, Iso, Suzuki, Takeyama, Shintani, Hashimoto, Baba, Shioda: "Cardioprotective effect of endogenous pituitary adenylate cyclase-activating polypeptide on Doxorubicin-induced cardiomyopathy in mice." in: Circulation journal : official journal of the Japanese Circulation Society, Vol. 74, Issue 6, pp. 1183-90, 2010 (PubMed).

Yamada, Ryo, Tai, Tamaki, Inoue, Mishima, Tsubota, Saito: "Evaluation of therapeutic effects of astaxanthin on impairments in salivary secretion." in: Journal of clinical biochemistry and nutrition, Vol. 47, Issue 2, pp. 130-7, 2010 (PubMed).

Herbas, Ueta, Ichikawa, Chiba, Ishibashi, Shichiri, Fukumoto, Yokoyama, Takeya, Xuan, Arai, Suzuki: "Alpha-tocopherol transfer protein disruption confers resistance to malarial infection in mice." in: Malaria journal, Vol. 9, pp. 101, 2010 (PubMed).

Raz, Zhang, Zhou, Han, Gulati, Yang, Yang, Wang, Brann: "Role of Rac1 GTPase in NADPH oxidase activation and cognitive impairment following cerebral ischemia in the rat." in: PLoS ONE, Vol. 5, Issue 9, pp. e12606, 2010 (PubMed).

Du, Wooten, Gearing, Wooten: "Age-associated oxidative damage to the p62 promoter: implications for Alzheimer disease." in: Free radical biology & medicine, Vol. 46, Issue 4, pp. 492-501, 2009 (PubMed).

Fujita, Miyachi, Tanaka, Takeo, Nakagawa, Kobayashi, Iwasa, Watanabe, Takei: "Iron overload is associated with hepatic oxidative damage to DNA in nonalcoholic steatohepatitis." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 18, Issue 2, pp. 424-32, 2009 (PubMed).

Background publications Iqbal, Okazaki, Okada: "Curcumin attenuates oxidative damage in animals treated with a renal carcinogen, ferric nitrilotriacetate (Fe-NTA): implications for cancer prevention." in: Molecular and cellular biochemistry, Vol. 324, Issue 1-2, pp. 157-64, 2009 (PubMed).

Song, Li, Ootsuyama, Kasai, Kawai, Ohta, Eguchi, Yamato, Matsumoto, Yoshida, Ogawa: "Urea, the most abundant component in urine, cross-reacts with a commercial 8-OH-dG ELISA kit and contributes to overestimation of urinary 8-OH-dG." in: Free radical biology & medicine, Vol. 47, Issue 1, pp. 41-6, 2009 (PubMed).

Sakano, Takahashi, Wang, Sauriasari, Takemoto, Kanbara, Sato, Takigawa, Takaki, Ogino: "Plasma 3-nitrotyrosine, urinary 8-isoprostane and 8-OHdG among healthy Japanese people." in: Free radical research, Vol. 43, Issue 2, pp. 183-92, 2009 (PubMed).

Sato, Harada, Ikeda, Fijii, Sasaki, Zen, Nakanuma: "Hepatic stellate cells are activated around central scars of focal nodular hyperplasia of the liver--a potential mechanism of central scar formation." in: Human pathology, Vol. 40, Issue 2, pp. 181-8, 2009 (PubMed).

Yamazaki, Miwa, Toyokuni, Nemoto, Oriyanhan, Takaba, Saji, Marui, Nishina, Ikeda, Komeda: "Effect of edaravone, a novel free radical scavenger, supplemented to cardioplegia on myocardial function after cardioplegic arrest: in vitro study of isolated rat heart." in: Heart and vessels, Vol. 24, Issue 3, pp. 228-35, 2009 (PubMed).

Herbas, Thekisoe, Inoue, Xuan, Arai, Suzuki: "The effect of alpha-tocopherol transfer protein gene disruption on Trypanosoma congolense infection in mice." in: Free radical biology & medicine, Vol. 47, Issue 10, pp. 1408-13, 2009 (PubMed).

Min, Park, Kim, Lee, Ha, Park: "Effect of oxidative stress on birth sizes: consideration of window from mid pregnancy to delivery." in: Placenta, Vol. 30, Issue 5, pp. 418-23, 2009 (PubMed).

Silva, Rosales, Biswas, Lopes de Faria, Lopes de Faria: "Diabetic retinal neurodegeneration is associated with mitochondrial oxidative stress and is improved by an angiotensin receptor blocker in a model combining hypertension and diabetes." in: Diabetes, Vol. 58, Issue 6, pp. 1382-90, 2009 (PubMed).