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8-Hydroxyguanosine (8-OHDG) antibody

Details for Product No. ABIN335420, Supplier: Login to see
Clonality (Clone)
Monoclonal ()
Immunohistochemistry (IHC), ELISA
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Immunogen 8-OHdG-conjugated Keyhole Limpet Hemocyanin
Clone N45-1
Isotype IgG1 kappa
Specificity 8-OHDG in all species. No observable crossreactivity for the following analogues: guanosine (G), 7methylG, 6SHG, 8bromoG, dA, dC, dT, dI, dU, dG, O6methyldG, 8OHdA, guanine (Gua), O6methylGua, 8OHGua, uric acid, Urea, creatine, creatinine Slight but minimal (<1 % ) crossreactivity for the following analogues: 8sulfhydrylG and 8OHG 1
Purification Prepared as ascites then ammonium sulphate purified
Alternative Name 8 Hydroxyguanosine (8-OHDG)
Target Type Chemical
Background 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical, singlet oxygen and direct photodynamic action. Anti 8-OHdG monoclonal antibody (clone N45.1) is highly specific for 8-OHdG, and is suitable for immunohistochemistry. Cross reactivity have been checked for 8-OHdG analogues (guanosine(G),7-methyl-G, 6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG, 8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G).
Research Area DNA/RNA
Application Notes Tested for IHC and ELISA. User should determine optimum titer for each application.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized as 100 ug/mL IgG in10 mM Phosphate buffered saline, pH7.4 containing 1.0 % BSA.
Storage -20 °C
Supplier Images
 image for anti-8-Hydroxyguanosine (8-OHDG) antibody (ABIN335420) Epidermis from hairless mouse by chronic UVB irradiation after 4 weeks of treatment s...
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Kato, Kobune, Nakamura et al.: "Normalization of elevated hepatic 8-hydroxy-2'-deoxyguanosine levels in chronic hepatitis C patients by phlebotomy and low iron diet." in: Cancer research, Vol. 61, Issue 24, pp. 8697-702, 2001 (PubMed).

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