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8-Hydroxyguanosine (8-OHDG) antibody

Details for Product No. ABIN335420
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Antigen
Reactivity
Chemical
(30), (1), (1), (1), (1)
Host
Mouse
(17), (14)
Clonality (Clone)
Monoclonal ()
Application
Immunohistochemistry (IHC), ELISA
(28), (15), (10), (5), (4), (3), (2), (2), (2), (2)
Pubmed 58 references available
Quantity 20 µg
Options
Shipping to United States (Change)
Availability Will be delivered in 7 to 8 Business Days
Catalog No. ABIN335420
396.19 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen 8-OHdG-conjugated Keyhole Limpet Hemocyanin
Clone N45-1
Isotype IgG1 kappa
Specificity 8-OHDG in all species. No observable crossreactivity for the following analogues: guanosine (G), 7methylG, 6SHG, 8bromoG, dA, dC, dT, dI, dU, dG, O6methyldG, 8OHdA, guanine (Gua), O6methylGua, 8OHGua, uric acid, Urea, creatine, creatinine Slight but minimal (<1%) crossreactivity for the following analogues: 8sulfhydrylG and 8OHG 1
Purification Prepared as ascites then ammonium sulphate purified
Alternative Name 8 Hydroxyguanosine (8-OHDG)
Target Type Chemical
Background 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical, singlet oxygen and direct photodynamic action. Anti 8-OHdG monoclonal antibody (clone N45.1) is highly specific for 8-OHdG, and is suitable for immunohistochemistry. Cross reactivity have been checked for 8-OHdG analogues (guanosine(G),7-methyl-G, 6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG, 8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G).
Research Area DNA/RNA
Application Notes Tested for IHC and ELISA. User should determine optimum titer for each application.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized as 100 ug/mL IgG in10mM Phosphate buffered saline, pH7.4 containing 1.0% BSA.
Storage -20 °C
Supplier Images
anti-8-Hydroxyguanosine (8-OHDG) antibody Epidermis from hairless mouse by chronic UVB irradiation after 4 weeks of treatment stained with N45.1. (Y.Hattori, et.al.: J Invest Dermatol 107, p733-737, 1997)
Product cited in: Hattori, Nishigori, Tanaka et al.: "8-hydroxy-2'-deoxyguanosine is increased in epidermal cells of hairless mice after chronic ultraviolet B exposure." in: The Journal of investigative dermatology, Vol. 107, Issue 5, pp. 733-7, 1996 (PubMed).

Toyokuni, Tanaka, Hattori et al.: "Quantitative immunohistochemical determination of 8-hydroxy-2'-deoxyguanosine by a monoclonal antibody N45.1: its application to ferric nitrilotriacetate-induced renal carcinogenesis model." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 76, Issue 3, pp. 365-74, 1997 (PubMed).

Tanaka, Nishiyama, Okada et al.: "Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 77, Issue 2, pp. 145-55, 1997 (PubMed).

Koyama, Wada, Sakuraba et al.: "Accelerated loss of islet beta cells in sucrose-fed Goto-Kakizaki rats, a genetic model of non-insulin-dependent diabetes mellitus." in: The American journal of pathology, Vol. 153, Issue 2, pp. 537-45, 1998 (PubMed).

Kondo, Toyokuni, Iwasa et al.: "Persistent oxidative stress in human colorectal carcinoma, but not in adenoma." in: Free radical biology & medicine, Vol. 27, Issue 3-4, pp. 401-10, 1999 (PubMed).

Kawashima, Bando, Nakamura et al.: "Cytoprotective effects of nitroglycerin in ischemia-reperfusion-induced lung injury." in: American journal of respiratory and critical care medicine, Vol. 161, Issue 3 Pt 1, pp. 935-43, 2000 (PubMed).

Ahmed, Ueda, Nikaido et al.: "High levels of 8-hydroxy-2'-deoxyguanosine appear in normal human epidermis after a single dose of ultraviolet radiation." in: The British journal of dermatology, Vol. 140, Issue 2, pp. 226-31, 2000 (PubMed).

Yamagami, Yamamoto, Kume et al.: "Formation of 8-hydroxy-2'-deoxyguanosine and 4-hydroxy-2-nonenal-modified proteins in rat liver after ischemia-reperfusion: distinct localization of the two oxidatively modified products." in: Antioxidants & redox signaling, Vol. 2, Issue 1, pp. 127-36, 2001 (PubMed).

Miyamoto, Kishimoto, Shioji et al.: "Difference in thioredoxin expression in viral myocarditis in inbred strains of mice." in: Japanese circulation journal, Vol. 65, Issue 6, pp. 561-4, 2001 (PubMed).

Gottschling, Maronpot, Hailey et al.: "The role of oxidative stress in indium phosphide-induced lung carcinogenesis in rats." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 64, Issue 1, pp. 28-40, 2001 (PubMed).

Aoshiba, Tamaoki, Nagai: "Acute cigarette smoke exposure induces apoptosis of alveolar macrophages." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 281, Issue 6, pp. L1392-401, 2001 (PubMed).

Kato, Kobune, Nakamura et al.: "Normalization of elevated hepatic 8-hydroxy-2'-deoxyguanosine levels in chronic hepatitis C patients by phlebotomy and low iron diet." in: Cancer research, Vol. 61, Issue 24, pp. 8697-702, 2001 (PubMed).

Liardet, Scaletta, Panizzon et al.: "Protection against pyrimidine dimers, p53, and 8-hydroxy-2'-deoxyguanosine expression in ultraviolet-irradiated human skin by sunscreens: difference between UVB + UVA and UVB alone sunscreens." in: The Journal of investigative dermatology, Vol. 117, Issue 6, pp. 1437-41, 2002 (PubMed).

Tanito, Nishiyama, Tanaka et al.: "Change of redox status and modulation by thiol replenishment in retinal photooxidative damage." in: Investigative ophthalmology & visual science, Vol. 43, Issue 7, pp. 2392-400, 2002 (PubMed).

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Tanito, Takanashi, Kaidzu et al.: "Cytoprotective effects of rebamipide and carteolol hydrochloride against ultraviolet B-induced corneal damage in mice." in: Investigative ophthalmology & visual science, Vol. 44, Issue 7, pp. 2980-5, 2003 (PubMed).

Yamakoshi, Otsuka, Sano et al.: "Lightening effect on ultraviolet-induced pigmentation of guinea pig skin by oral administration of a proanthocyanidin-rich extract from grape seeds." in: Pigment cell research / sponsored by the European Society for Pigment Cell Research and the International Pigment Cell Society, Vol. 16, Issue 6, pp. 629-38, 2003 (PubMed).

Tam, Gao, Leung et al.: "Androgenic regulation of oxidative stress in the rat prostate: involvement of NAD(P)H oxidases and antioxidant defense machinery during prostatic involution and regrowth." in: The American journal of pathology, Vol. 163, Issue 6, pp. 2513-22, 2003 (PubMed).

Ichiseki, Kaneuji, Katsuda et al.: "DNA oxidation injury in bone early after steroid administration is involved in the pathogenesis of steroid-induced osteonecrosis." in: Rheumatology (Oxford, England), Vol. 44, Issue 4, pp. 456-60, 2005 (PubMed).

Nagotani, Hayashi, Sato et al.: "Reduction of cerebral infarction in stroke-prone spontaneously hypertensive rats by statins associated with amelioration of oxidative stress." in: Stroke; a journal of cerebral circulation, Vol. 36, Issue 3, pp. 670-2, 2005 (PubMed).

Zhang, Komine-Kobayashi, Tanaka et al.: "Edaravone reduces early accumulation of oxidative products and sequential inflammatory responses after transient focal ischemia in mice brain." in: Stroke; a journal of cerebral circulation, Vol. 36, Issue 10, pp. 2220-5, 2005 (PubMed).

Piao, Ma, Hiraku et al.: "Oxidative DNA damage in relation to neurotoxicity in the brain of mice exposed to arsenic at environmentally relevant levels." in: Journal of occupational health, Vol. 47, Issue 5, pp. 445-9, 2005 (PubMed).

Albrecht, Knaapen, Becker et al.: "The crucial role of particle surface reactivity in respirable quartz-induced reactive oxygen/nitrogen species formation and APE/Ref-1 induction in rat lung." in: Respiratory research, Vol. 6, pp. 129, 2005 (PubMed).

Suzuki, Ueta, Inoue et al.: "Beneficial effect of erythropoietin administration on murine infection with Trypanosoma congolense." in: The American journal of tropical medicine and hygiene, Vol. 74, Issue 6, pp. 1020-5, 2006 (PubMed).

Kaewamatawong, Shimada, Okajima et al.: "Acute and subacute pulmonary toxicity of low dose of ultrafine colloidal silica particles in mice after intratracheal instillation." in: Toxicologic pathology, Vol. 34, Issue 7, pp. 958-65, 2006 (PubMed).

Choi, Ryu, Kim et al.: "Modulation of the purinergic P2X7 receptor attenuates lipopolysaccharide-mediated microglial activation and neuronal damage in inflamed brain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 18, pp. 4957-68, 2007 (PubMed).

Kurimoto, Kawano, Tsuji et al.: "Thioredoxin may exert a protective effect against tissue damage caused by oxidative stress in salivary glands of patients with Sjögren's syndrome." in: The Journal of rheumatology, Vol. 34, Issue 10, pp. 2035-43, 2007 (PubMed).

Betsuyaku, Hamamura, Hata et al.: "Bronchiolar chemokine expression is different after single versus repeated cigarette smoke exposure." in: Respiratory research, Vol. 9, pp. 7, 2008 (PubMed).

Lee, Hsu, Wang et al.: "Chemopreventive effect of selenium and Chinese medicinal herbs on N-nitrosobis(2-oxopropyl)amine-induced hepatocellular carcinoma in Syrian hamsters." in: Liver international : official journal of the International Association for the Study of the Liver, Vol. 28, Issue 6, pp. 841-55, 2008 (PubMed).

Umekawa, Sugiyama, Kihira et al.: "Overexpression of thioredoxin-1 reduces oxidative stress in the placenta of transgenic mice and promotes fetal growth via glucose metabolism." in: Endocrinology, Vol. 149, Issue 8, pp. 3980-8, 2008 (PubMed).

Chigurupati, Son, Hyun et al.: "Lifelong running reduces oxidative stress and degenerative changes in the testes of mice." in: The Journal of endocrinology, Vol. 199, Issue 2, pp. 333-41, 2008 (PubMed).

Fujihara, Nagai, Sussan et al.: "Chronic cigarette smoke causes oxidative damage and apoptosis to retinal pigmented epithelial cells in mice." in: PLoS ONE, Vol. 3, Issue 9, pp. e3119, 2008 (PubMed).

Ribaldo, Souza, Biswas et al.: "Green tea (Camellia sinensis) attenuates nephropathy by downregulating Nox4 NADPH oxidase in diabetic spontaneously hypertensive rats." in: The Journal of nutrition, Vol. 139, Issue 1, pp. 96-100, 2008 (PubMed).

Du, Wooten, Gearing et al.: "Age-associated oxidative damage to the p62 promoter: implications for Alzheimer disease." in: Free radical biology & medicine, Vol. 46, Issue 4, pp. 492-501, 2009 (PubMed).

Fujita, Miyachi, Tanaka et al.: "Iron overload is associated with hepatic oxidative damage to DNA in nonalcoholic steatohepatitis." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 18, Issue 2, pp. 424-32, 2009 (PubMed).

Celino, Yamaguchi, Miura et al.: "Arsenic inhibits in vitro spermatogenesis and induces germ cell apoptosis in Japanese eel (Anguilla japonica)." in: Reproduction (Cambridge, England), Vol. 138, Issue 2, pp. 279-87, 2009 (PubMed).

Ueno, Torimura, Nakamura et al.: "Epigallocatechin-3-gallate improves nonalcoholic steatohepatitis model mice expressing nuclear sterol regulatory element binding protein-1c in adipose tissue." in: International journal of molecular medicine, Vol. 24, Issue 1, pp. 17-22, 2009 (PubMed).

Freeman, Parvizi, Della Valle et al.: "Reactive oxygen and nitrogen species induce protein and DNA modifications driving arthrofibrosis following total knee arthroplasty." in: Fibrogenesis & tissue repair, Vol. 2, Issue 1, pp. 5, 2009 (PubMed).

Rabbani, Mi, Zhang et al.: "Hypoxia inducible factor 1alpha signaling in fractionated radiation-induced lung injury: role of oxidative stress and tissue hypoxia." in: Radiation research, Vol. 173, Issue 2, pp. 165-74, 2010 (PubMed).

Mori, Nakamachi, Ohtaki et al.: "Cardioprotective effect of endogenous pituitary adenylate cyclase-activating polypeptide on Doxorubicin-induced cardiomyopathy in mice." in: Circulation journal : official journal of the Japanese Circulation Society, Vol. 74, Issue 6, pp. 1183-90, 2010 (PubMed).

Herbas, Ueta, Ichikawa et al.: "Alpha-tocopherol transfer protein disruption confers resistance to malarial infection in mice." in: Malaria journal, Vol. 9, pp. 101, 2010 (PubMed).

Calveley, Jelveh, Langan et al.: "Genistein can mitigate the effect of radiation on rat lung tissue." in: Radiation research, Vol. 173, Issue 5, pp. 602-11, 2010 (PubMed).

Raz, Zhang, Zhou et al.: "Role of Rac1 GTPase in NADPH oxidase activation and cognitive impairment following cerebral ischemia in the rat." in: PLoS ONE, Vol. 5, Issue 9, pp. e12606, 2010 (PubMed).

Yamada, Ryo, Tai et al.: "Evaluation of therapeutic effects of astaxanthin on impairments in salivary secretion." in: Journal of clinical biochemistry and nutrition, Vol. 47, Issue 2, pp. 130-7, 2010 (PubMed).

Sun, Sugiyama, Masuda et al.: "Expressions of protein oxidation markers, dityrosine and advanced oxidation protein products in acetaminophen-induced liver injury in rats." in: The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Vol. 73, Issue 9, pp. 1185-90, 2011 (PubMed).

Toyokuni, Hirao, Wada et al.: "Age- and sun exposure-dependent differences in 8-hydroxy-2'-deoxyguanosine and N-(carboxymethyl)lysine in human epidermis." in: Journal of clinical biochemistry and nutrition, Vol. 49, Issue 2, pp. 121-4, 2011 (PubMed).

Baila, Ohno, Nagamoto et al.: "Tetomilast attenuates elastase-induced pulmonary emphysema through inhibition of oxidative stress in rabbits." in: Biological & pharmaceutical bulletin, Vol. 35, Issue 4, pp. 494-502, 2012 (PubMed).

Sintara, Thong-Ngam, Patumraj et al.: "Curcumin attenuates gastric cancer induced by N-methyl-N-nitrosourea and saturated sodium chloride in rats." in: Journal of biomedicine & biotechnology, Vol. 2012, pp. 915380, 2012 (PubMed).

General Ihara, Toyokuni, Uchida et al.: "Hyperglycemia causes oxidative stress in pancreatic beta-cells of GK rats, a model of type 2 diabetes." in: Diabetes, Vol. 48, Issue 4, pp. 927-32, 1999 (PubMed).

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Sakano, Takahashi, Wang et al.: "Plasma 3-nitrotyrosine, urinary 8-isoprostane and 8-OHdG among healthy Japanese people." in: Free radical research, Vol. 43, Issue 2, pp. 183-92, 2009 (PubMed).

Song, Li, Ootsuyama et al.: "Urea, the most abundant component in urine, cross-reacts with a commercial 8-OH-dG ELISA kit and contributes to overestimation of urinary 8-OH-dG." in: Free radical biology & medicine, Vol. 47, Issue 1, pp. 41-6, 2009 (PubMed).

Silva, Rosales, Biswas et al.: "Diabetic retinal neurodegeneration is associated with mitochondrial oxidative stress and is improved by an angiotensin receptor blocker in a model combining hypertension and diabetes." in: Diabetes, Vol. 58, Issue 6, pp. 1382-90, 2009 (PubMed).

Min, Park, Kim et al.: "Effect of oxidative stress on birth sizes: consideration of window from mid pregnancy to delivery." in: Placenta, Vol. 30, Issue 5, pp. 418-23, 2009 (PubMed).

Yamazaki, Miwa, Toyokuni et al.: "Effect of edaravone, a novel free radical scavenger, supplemented to cardioplegia on myocardial function after cardioplegic arrest: in vitro study of isolated rat heart." in: Heart and vessels, Vol. 24, Issue 3, pp. 228-35, 2009 (PubMed).

Herbas, Thekisoe, Inoue et al.: "The effect of alpha-tocopherol transfer protein gene disruption on Trypanosoma congolense infection in mice." in: Free radical biology & medicine, Vol. 47, Issue 10, pp. 1408-13, 2009 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN335420
396.19 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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