CD81 Molecule (CD81) antibody (PE)

Antigen: CD81 Molecule (CD81)

Clonality: Monoclonal (M38)

Host: Mouse

Reactivity: Human, Cat (Feline), Rabbit

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN343728

Additional Information

Alternative name: CD81

Immunogen: MOLT-4 (human T-ALL cell line)

Isotype: IgG1

Clone: M38

Description: CD81 (TAPA-1), a member of the tetraspanin family, is expressed on virtually all nucleatedcells, but above all on germinal center B cells. CD81 forms complexes with other tetraspaninproteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion,morphology, activation, proliferation and differentiation of B, T and other cells e.g. inmuscles CD81 promotes cell fusion and myotube maintenance. CD81 has been alsoidentified as a receptor for the hepatitis C virus.

Specificity: The antibody M38 reacts with CD81, a 25 kDa member of the tetraspanin family, expressedon majority of cells.

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Fukudome, Furuse, Imai et al.: "Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen in HTLV-1-positive T cells." in: Journal of virology, Vol. 66, Issue 3, pp. 1394-401, 1992 (PubMed).

Imai, Kakizaki, Nishimura et al.: "Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 3, pp. 1229-39, 1995 (PubMed).

Imai, Yoshie: "C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 6470-81, 1994 (PubMed).

Escola, Kleijmeer, Stoorvogel et al.: "Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes." in: The Journal of biological chemistry, Vol. 273, Issue 32, pp. 20121-7, 1998 (PubMed).