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NG & NG-NG-Methyl Arginine antibody

Details for Product No. ABIN346795, Supplier: Log in to see
All Species
Clonality (Clone)
Monoclonal ()
Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
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Immunogen Modified methyl arginine
Clone [7E6]
Isotype IgG1 kappa
Fragment Chain kappa
Specificity Reacts with all structure of methylated arginine including free and bound. Cross reacts with free and bound NG - Monomethyl Arginine. No cross reaction with free or bound Arginine. Ability to fix complement : unknown
Purification Purified (protein A)
Application Notes

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
- ChIP : 1/100 to 1/10 000 - WB : 1/500 to 1/5 000

Restrictions For Research Use only
Format Lyophilized
Reconstitution Must be reconstituted in distilled water.
Concentration 1 mg/mL
Buffer Tris 0,1M, glycine 0,1M, sucrose 2 %
Storage 4 °C,-20 °C
Storage Comment Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.
Expiry Date 24 months
Product cited in: Abboud, Storey: "Novel control of lactate dehydrogenase from the freeze tolerant wood frog: role of posttranslational modifications." in: PeerJ, Vol. 1, pp. e12, 2013

Ong, Mittler, Mann: "Identifying and quantifying in vivo methylation sites by heavy methyl SILAC." in: Nature methods, Vol. 1, Issue 2, pp. 119-26, 2005

Background publications Duong, Filipowicz, Tripodi, La Monica, Heim: "Hepatitis C virus inhibits interferon signaling through up-regulation of protein phosphatase 2A." in: Gastroenterology, Vol. 126, Issue 1, pp. 263-77, 2003

Fabbrizio, El Messaoudi, Polanowska, Paul, Cook, Lee, Negre, Rousset, Pestka, Le Cam, Sardet: "Negative regulation of transcription by the type II arginine methyltransferase PRMT5." in: EMBO reports, Vol. 3, Issue 7, pp. 641-5, 2002

Li, Park, Kilburn, Jelinek, Henschen-Edman, Aswad, Stallcup, Laird-Offringa: "Lipopolysaccharide-induced methylation of HuR, an mRNA-stabilizing protein, by CARM1. Coactivator-associated arginine methyltransferase." in: The Journal of biological chemistry, Vol. 277, Issue 47, pp. 44623-30, 2002

Zhu, Mustelin, David: "Arginine methylation of STAT1 regulates its dephosphorylation by T cell protein tyrosine phosphatase." in: The Journal of biological chemistry, Vol. 277, Issue 39, pp. 35787-90, 2002

Mowen, Tang, Zhu, Schurter, Shuai, Herschman, David: "Arginine methylation of STAT1 modulates IFNalpha/beta-induced transcription." in: Cell, Vol. 104, Issue 5, pp. 731-41, 2001

Rho, Choi, Seong, Choi, Im: "The arginine-1493 residue in QRRGRTGR1493G motif IV of the hepatitis C virus NS3 helicase domain is essential for NS3 protein methylation by the protein arginine methyltransferase 1." in: Journal of virology, Vol. 75, Issue 17, pp. 8031-44, 2001