NG & NG-NG-Methyl Arginine antibody

Details for Product No. ABIN346795
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Antigen
Reactivity
All Species
(2), (1)
Host
Mouse
(3)
Clonality (Clone)
Monoclonal ()
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP)
(3), (1), (1)
Pubmed 8 references available
Quantity 100 μg
Options
Shipping to United States ( )
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Request Want additional data for this product?

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Immunogen Modified methyl arginine
Clone [7E6]
Isotype IgG1 kappa
Cross-Reactivity (Details) Reacts with all structure of methylated arginine including free and bound. Cross reacts with free and bound NG - Monomethyl Arginine. No cross reaction with free or bound Arginine. Ability to fix complement: unknown
Purification Purified (protein A)
Application Notes Optimal dilution should be determined by the end user.
The following are guidelines only:
IHC : 1/100 to 1/10 000, WB : 1/500 to 1/5 000
Restrictions For Research Use only
Format Lyophilized
Reconstitution Must be reconstituted in distilled water.
Concentration 1 mg/mL
Buffer Tris 0,1 M, glycine 0,1 M, sucrose 2 %
Storage 4 °C/-20 °C
Storage Comment Lyophilized powder stable for a minimum of 2 years at -20 °C. Store reconstituted antibodies at +4 °C. For extended periods store in aliquots at -20 °C. Antibodies are guaranteed for 6 month from date of receipt.
Expiry Date 24 months
Product cited in: Abboud, Storey: "Novel control of lactate dehydrogenase from the freeze tolerant wood frog: role of posttranslational modifications." in: PeerJ, Vol. 1, pp. e12, 2013 (PubMed).

Ong, Mittler, Mann: "Identifying and quantifying in vivo methylation sites by heavy methyl SILAC." in: Nature methods, Vol. 1, Issue 2, pp. 119-26, 2005 (PubMed).

Duong, Filipowicz, Tripodi et al.: "Hepatitis C virus inhibits interferon signaling through up-regulation of protein phosphatase 2A." in: Gastroenterology, Vol. 126, Issue 1, pp. 263-77, 2003 (PubMed).

Mowen, Tang, Zhu et al.: "Arginine methylation of STAT1 modulates IFNalpha/beta-induced transcription." in: Cell, Vol. 104, Issue 5, pp. 731-41, 2001 (PubMed).

General Li, Park, Kilburn et al.: "Lipopolysaccharide-induced methylation of HuR, an mRNA-stabilizing protein, by CARM1. Coactivator-associated arginine methyltransferase." in: The Journal of biological chemistry, Vol. 277, Issue 47, pp. 44623-30, 2002 (PubMed).

Zhu, Mustelin, David: "Arginine methylation of STAT1 regulates its dephosphorylation by T cell protein tyrosine phosphatase." in: The Journal of biological chemistry, Vol. 277, Issue 39, pp. 35787-90, 2002 (PubMed).

Fabbrizio, El Messaoudi, Polanowska et al.: "Negative regulation of transcription by the type II arginine methyltransferase PRMT5." in: EMBO reports, Vol. 3, Issue 7, pp. 641-5, 2002 (PubMed).

Rho, Choi, Seong et al.: "The arginine-1493 residue in QRRGRTGR1493G motif IV of the hepatitis C virus NS3 helicase domain is essential for NS3 protein methylation by the protein arginine methyltransferase 1." in: Journal of virology, Vol. 75, Issue 17, pp. 8031-44, 2001 (PubMed).

Validation Images
Catalog No. ABIN346795
432.91 $
Plus shipping costs $45.00
Quantity
Price
100 μg
432.91 $

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