Solute Carrier Family 1 (Neuronal/epithelial High Affinity Glutamate Transporter, System Xag), Member 1 (SLC1A1) (Extracellular Domain) antibody

Antigen: Solute Carrier Family 1 (Neuronal/epithelial High Affinity Glutamate Transporter, System Xag), Member 1 (SLC1A1)

Synonyms: EAAC1, EAAT3, EAAC2, MEAAC1, D130048G10Rik, Eaac1, Eaat3, REAAC1, GB16911, zgc:91959

Binding Site: Extracellular Domain

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350316

Additional Information

Alternative name: Excitatory amino acid transporter 3 (EAAT3, EAAC1)

UniProt: P43005

Immunogen: A synthetic peptide from extracellular domain of human Excitatory amino acid transporter 3 (EAAT3, EAAC1) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shres 96% identity with rat and mouse sequence.

Format: Lyophilized

Isotype: IgG

Description: FUNCTION: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5. Defects in SLC1A1 may be a cause of dicarboxylicamino aciduria; also known as glutamate-aspartate transport defect. This is as defect in renal and probably intestinal transport of glutamic and aspartic acids and is associated with moderate hyperprolinemia. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers). Also known as: Sodium-dependent glutamate/aspartate transporter 3, Excitatory amino-acid carrier 1, Neuronal and epithelial glutamate transporter, Solute carrier family 1 member 1, EAAC1, EAAT3, SLC1A1.

Specificity: Appears to be specific for EAAT3.

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Arriza, Fairman, Wadiche et al.: "Functional comparisons of three glutamate transporter subtypes cloned from human motor cortex." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 14, Issue 9, pp. 5559-69, 1994 (PubMed).

Shashidharan, Huntley, Meyer et al.: "Neuron-specific human glutamate transporter: molecular cloning, characterization and expression in human brain." in: Brain research, Vol. 662, Issue 1-2, pp. 245-50, 1995 (PubMed).

Kanai, Stelzner, Nussberger et al.: "The neuronal and epithelial human high affinity glutamate transporter. Insights into structure and mechanism of transport." in: The Journal of biological chemistry, Vol. 269, Issue 32, pp. 20599-606, 1994 (PubMed).

Veenstra-VanderWeele, Kim, Gonen et al.: "Genomic organization of the SLC1A1/EAAC1 gene and mutation screening in early-onset obsessive-compulsive disorder." in: Molecular psychiatry, Vol. 6, Issue 2, pp. 160-7, 2001 (PubMed).

Humphray, Oliver, Hunt et al.: "DNA sequence and analysis of human chromosome 9." in: Nature, Vol. 429, Issue 6990, pp. 369-74, 2004 (PubMed).